Splenic macrophages

Ladies, G.S., et al., Generation of 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene by murine splenic macrophages, Toxicol. Appl. Pharmacol., 115, 72, 1992. 

Lee, J.J. and Battles, A.H., Lead toxicity via arachidonate signal transduction to growth responses in the splenic macrophages, Environ. Res. 67, 209, 1994. 

TCDD to young adult male Leeds strain rats (Badesha et al. 1995). A 30-day exposure to approximately 0. 1 g/kg/day (or a total dose of approximately 3 g/kg) resulted in an exposure duration-dependent reduction of in vitro lipopolysaccharide-induced production of interleukin-1 in cultures of their splenic macrophages. A 180-day exposure to approximately 0.017 g/kg/day suppressed the production of interleukin-2 by either concanavalin A or phorbol ester/calcium ionophore stimulation, and reduced the lectin-induced proliferation of splenic T cells. The authors concluded that exposure to a low dietary dose of 2,3,7,8-TCDD suppresses the functions of several T-cell subsets. The highest NOAEL value for immunological effects (decreased thymus weight) was 0.0007 g/kg/day... 

Rahim RT, Meissler Jr JJ, Zhang L, Adler MW, Rogers TJ, Eisenstein TK (2003) Withdrawal from morphine in mice snppresses splenic macrophage fnnction, cytokine production, and costimnlatory molecnles. J Nenroimmnnol 144 16-27. 

Rahim RT, Meissler Jr JJ, Adler MW, Eisenstein TK (2005) Splenic macrophages and B-cells mediate immnnosnppression following abrnpt withdrawal from morphine. J Lenkoc Biol 78 1185-1191. 

Brinkmann V, Kaufmann SH, Simon MM, et al Role of Macrophages in Malaria 02 Metabolite Production and Phagocytosis by Splenic Macrophages During Lethal Plasmodium berghei and Self-limiting Plasmodium yoelii Infection in Mice. Infect. Immun. 1984 44(3) 7434. 

Shibata Y, Bautista AP, Pennington SN, et al. 1987. Eicosanoid production by peritoneal and splenic macrophages in mice depleted of bone marrow by 89Sr. Am J Physiol 127 75-82. 

Bite cells are RBCs with bites of cytoplasm being removed by splenic macrophages. Bite cells may be seen in G6PD deficiency. 

The Listeria monocytogenes host resistance model is controlled primarily in the liver and spleen. The Listeria monocytogenes systemic infection assay is useful primarily to evaluate adverse effects on neutrophils and Kupffer cells of the liver and splenic macrophages and neutrophils. NK cells and T lymphocytes also play a role in bacterial clearance. The Listeria monocytogenes host... 

Gallium arsenide has been shown to be an immunosuppressant. When administered intratracheally to mice at concentrations between 50 and 200 mg kg GaAs suppressed the in-vivo antibody-forming cell (AFC) response to sheep erythrocytes (SRBC), and interfered with antigen processing that is essential for CD4+ T-cell stimulation by splenic macrophages (Sikor-ski etal. 1991, Lewis TA etal. 1996). The arsenic moiety appears to be responsible... 

Lewis TA, Munson AE and McCoy KL (1996) Gallium arsenide selectively suppresses antigen processing by splenic macrophages for CD4-y- T Cell Activation. J Pharmacol Exp Ther 278 1244-1251. 

Fig. 188. Tubular invaginations in a splenic macrophage (block 4836) activated 16 weeks before by two portions of 0.05 ml complete Freund s adjuvant given intravenously at an interval of 24 h. Male Sprague-Dawley rat (Charles River, France) perfused under pentobarbital anaesthesia (30 mg/kg) from the abdominal aorta with 2.5 % glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4). Postfixation with 1 % osmium tetroxide in cacodylate buffer. Embedded in Epon 812 and sectioned at 50 nm. Lead citrate and uranyl acetate. Film 214/84...

Studies in weaning rats, in which the spleen was stained with the Prussian blue technique, indicate that the uptake of hemosiderin by splenic macrophage does not occur until adult-age histological development is achieved (58-60 days postpartum). Moreover, the ability of the spleen macrophage to store iron increases with the age of the animal. 

---