Sphingomyelin 2, 2

A few points should be noted for identification of SM species present in biological samples by using the recognized fragmentation pattern. 

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CiaH3 7N02,CH3-[CH2],2-CH CH.CH(0H). CH(NH2>-CH20H. a base, forming part of the molecules of sphingomyelins and the cerebrosides, from which it splits off on hydrolysis. It crystallizes in needles from ether. 

Anilinonaphthalenesulfonic acid ammonia salt (ANS reagent) fatty acids [112,113] lecithin/sphingomyelin [114, 115] cholesterol and its esters [116, 117] steroids, detergents, hydrocarbons [118,119] prenol, prenylquinones [120]... 

Ammonium sulfate phosphatidyl glycerol, sphingomyelin 10 mm at 280 °C densitometric in situ quantitation [199]... 

Detection and result The chromatogram was dried in a stream of cold air fo 2 min and sprayed three times with the spray reagent until it began to appeal transparent. The plate was dried in cold air after each spray step and finally heater to 120°C for 15 min. Lecithin hRf 15) and sphingomyelin hR( 5 — 10) appearet as dark blue zones on a yellow background (Fig. 1). 

Fig. 1 Reflectance scan of chromatogram tracks (A layer prewashed with mobile phase, with methanol) with 750 ng each substance per chromatogram zone. Sphingomyelin (1)] lecithin (2).

FIGURE 8.12 A structure and a space-filling model of a choline sphingomyelin formed from stearic acid. 

FIGURE 25.26 Glycosylceramides (such as galactosylceramide), gangUosides, and sphingomyelins are synthesized from ceramide in animals. 

Sphingomyelins are the second major group of phospholipids. These compounds have sphingosine or a related dihydroxyamine as their backbone and are particularly abundant in brain and nerve tissue, where they are a major constituent of the coating around nerve fibers. 

Phospholipids are important constituents of cell membranes and are of two kinds. Glycerophospholipids, such as phosphatidylcholine and phos-phatidylethanolamine, are closely related to fats in that they have a glycerol backbone esterified to two fatty acids (one saturated and one unsaturaled) and to one phosphate ester. Sphingomyelins have the amino alcohol sphingo-sine for their backbone. 

Sphingomyelin (Section 27.3) A phospholipid that has sphingosine as its backbone. 

Sphingomyelin, 1066-1067 Sphingosine, structure of, 1067 Spin density surface, allylic radical, 342... 

Signaling phospholipid derived from sphingomyelin by phospholipase action. Activates SIP receptors. 

Vitamins and lipids are often required for animal cells to grow in serum-free medium. Phosphoethanolamine and ethanolamine are key additives that facilitate the growth of the mammary tumor cell line 64024 (Kano-Sueoka and Errick, 1981). In addition, ethanolamine promotes the growth of human lymphocytes and mouse hybridoma cells. Short-term cultures of human diploid lung and foreskin fibroblasts grow in medium that includes among its supplements soybean lecithin, cholesterol, sphingomyelin, and vitamin E. 

Sphingomyelin 44, 89, 377, 378 Spironolactone 411 Spot diameter 78,131 Spray gun 81 Spraying... 

Ellens, H., Morselt, H., and Scherphof, G. (1981). In vivo fate of large unilamellar sphingomyelin-cholesterol Liposomes after intraperitoneal and intravenous injection into rats, Biochim. Biophys. Acta. 674, 10-18. 

Estep, T. N., Mouncastle, D. B., Barenholz, Y., Biltonen, L., and Thompson, T. E. (1979). Thermal behavior of synthetic sphingomyelin-cholesterol dispersions, Biochemistry. 18. 2112-2117. 

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