Sophora flavescens Matrine

The high-performance liquid chromatography (HPLC) determination of quinolizidine alkaloids in Radix Sophora flavescens was assisted by using tris(2,2 -bipyridyl)ruthenium(n) electrochemoluminescence <2004MI237>. Tandem HPLC-MS techniques have allowed the development of a sensitive and specific method for the determination of sophocarpine, matrine, and sophoridine in rabbit plasma <2005MI1595>. 

Isomatrine was isolated from Sophora flavescens Ait. (79,208). There are absorption bands of a lactam group at 1620 cm and a rrani-quinolizidine at 2740, 2760, and 2780 cm in the IR spectrum. Heating of isomatrine (29) in aqueous solution with Pt02/H2 results in epimerization, and a mixture of matrine... 

Sophora flavescens Ait. S. alopecurosides L. Ku Seng Gu Dong Zi (root) d-oxymatrine, d-sophoranol, cytisine, 1-anagyrine, 1-baptifoline, 1-methylcytisine, trifolirhizin, d-matrine, norkurarinone, kuraridin.36 Anthelmintic, antipruritic, treats irregular heart beat, eczema, acute dysentery, trichomoniasis. 

Cai et al. first used a chloroform-0.07 M sodium phosphate buffer (pH 6.4-6.5) solvent system for the separation of matrine and oxymatrine from Sophora flavescens, atropine, scopolamine, and hyoscyamine from Datura mete L. by HSCCC. Cooper et al. successfully used chloroform-0.2 M potassium phosphate buffer with an optimum pH value of each at 5.0, 5.6, 6.0, and 7.4 for the separation of pyrrolizidine alkaloids from various sources of Senecio douglasii var. longilobus, Trichodesma incanum, Symphytum spp. and Amsinckia tessellata, respectively. 

The roots of Sophora flavescens contains isomatrine (22), a stereoisomer of matrine that was epimerized into a mixture of matrine and allomatrine. X-ray analysis established the structure and absolute configuration of the new alkaloid. " Structure (23) has been proposed for lehmannine from Ammothamnus lehmanni as a result of spectral information and catalytic reduction of the alkaloid to matrine. ... 

In vitro tissue and cell cultures of lupin plants are not appropriate systems for the study of biosynthesis of lupin alkaloids, because the production ability by in vitro culture is rather low, i.e., 10 2 to lO times compared with that of differentiated plants. The production of the alkaloids of lupinine- and sparteine-groups by cell culture have been reported by us [59] and by Wink s group [60]. We have also successfully produced matrine in green callus culture and in multiple shoots of Sophora flavescens [61]. The producibility of matrine was positively correlated with the chloroplast formation. This indicates that the formation of carbon skeleton of matrine-type alkaloids also likely takes place in chloroplasts in plant cells as postulated in that of sparteine-type alkaloids [62]. 

Alkaloids of the matrine group have attracted considerable attention this year. Several alkaloids of this type were obtained previously from the roots of Sophora flavescens, and two new alkaloids have now been isolated from the above-ground part of the plant. One of these alkaloids is the fully conjugated pyridone 7,8-dehydrosophoramine (15), since reduction afforded (-)-sophoramine and the n.m.r. spectrum indicated the presence of a methylene group at C-17 and a methine proton at C-8. The structure of the other new alkaloid, 13,14-dehydrosophoridine (16), was established by X-ray analysis of its monohydrate, 0. 

Matrine and oxymatrine MISPE of herb sophora flavescens Haginaka etal., 2011... 

Funaya, N., Haglnaka, J. (2012). Matrlne- and oxymatrlne-lmprinted monodisperse polymers prepared by precipitation polymerization and their applications for the selective extraction of matrine-lype alkaloids from Sophora flavescens Alton, J ClTrgmaWgn 1248, 18-23. 

Matrine A Pm dissipation, release of cyt-c, ratio Bcl-2/Bax protein decreased caspase-3 activation. Human multiple myeloma cells Alkaloid isolated from the traditional Chinese herb Sophora flavescens. [141]... 

Sophora falvescens (Ku shen) contains a variety of matrine alkaloids, such as aloperine, cytosine, lehman-nine, matrine, oxymartine, oxysophocarpine, sophocar-pine, sophoramine, and sophoridine, the flavonoid kushenol, and the saponin sophoraflavoside. Ku shen is a Chinese herbal remedy made from the root of S. falvescens. And Qing luo yin, a Chinese herbal remedy for rheumatoid arthritis, is a combination of extracts of Dioscorea hypoglauca, Phellodendron amurense, Sinomenium acutum, and S. flavescens. 

Matrine (29) is a quinolizidine alkaloid isolated from several species of the genus Sophora (Fabaceae), mainly S. alopecuroides L. and S.flavescens Aiton. The latter has widely been used by traditional Chinese medicine, and its pharmacological properties were correlated to matrine contents [115, 116],... 

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