Somatic mutation/recombination test SMART

Another target of Vinca alkaloids seems to be DNA. Tiburi et ai. [184] showed that vinblastine, vincristine and vinorelbine all had significant genotoxicity, as assayed by the wing Somatic Mutation and Recombination Test (SMART) of Drosophila. All the three drugs caused increments in the incidence of mutational events and somatic recombination. 

Dose-dependent induction of chromosomal aberrations and sister chromatid exchanges were observed in human lymphocytes treated with dill leaf or seed essential oil. No significant effects of dill seed essential oil were observed in the Drosophila melanogaster somatic mutation and recombination test (SMART) in vivo (Lazutka et al. 2001). 

No mutagenic activity of an aqueous extract of linden was observed in the somatic mutation and recombination test (SMART) in Drosophila melanogaster (Romero-Jimenez et al. 2005). 

At present, several antigenotoxicity/genotoxicity assays which include the chromosome aberration (CA), micronucleus (MN), somatic mutation and recombination test (SMART), sister chromatid exchange (SCE) and the single-cell gel electrophoresis (SCGE) or COMET assays are available, and they are recommended to be used as a set for investigations. 

In a study of Villatoro-Pulido et al. [155], radish was grown on metal-contaminated or control soU, harvested, and fed to larvae of Drosophila melanogaster for which the somatic mutation and recombination test (SMART) in wings is well established. Radish roots of untreated soils were not genotoxic, the ones of metal-treated soils rally at highest concentrations and all shoots of metals-treated soils caused genotoxicity in the fruitfly. To learn more about the genotoxic potential of cadmium, like threshold values, in plants and their consumers is therefore important. 

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