Sodium dodecyl sulfate capillary electrophoresis separation

Guttman, A, On the Separation Mechanism of Capillary Sodium Dodecyl Sulfate-Gel Electrophoresis of Proteins, Electrophoresis 16, 611, 1995. 

Guttman, A. and Nolan, J., Comparison of the separation of proteins by sodium dodecyl sulfate-slab gel electrophoresis and capillary sodium dodecyl sulfate-gel electrophoresis, Anal. Biochem., 221, 285, 1994. 

JP Ou, STH Chang, WSB Yeung. Separation of bovine serum albumin and its monoclonal antibody from their immunocomplexes by sodium dodecyl sulfate-capillary gel electrophoresis and its application in capillary electrophoresis-based immunoassay. J Chromatogr B 731 389—394, 1999. 

Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis (SDS-CGE). SDS-CGE is a capillary-based version of SDS-polyacrylamide gel electrophoresis (SDS-PAGE) in the slab gel format, with advantages of shorter analysis times, ease of automation, and online detection and quantitation [26, 27]. In SDS-CGE, replaceable sieving polymers, such as linear polyacrylamide, poly (ethylene oxide), dextran, or pullulan, are used to achieve reproducible separations. These polymers permit the replacement of a separation matrix for each sample, thereby eliminating cross-contamination between samples and improving reproducibility. Best results are often obtained using chemically or dynamically coated capillaries. 

Corradini, D., Buffer additives other than the surfactant sodium dodecyl sulfate for protein separation by capillary electrophoresis, J. Chromatogr. B, 699, 21, 1997. 

M. Nakatani, A. Shibukawa, and T. Nakagawa. Separation mechanism of pul-lulan solution-filled capillary electrophoresis of sodium dodecyl sulfate-proteins. Electrophoresis, 17 (1996), 1584-1586. 

Dittman, M. M. and Rozing, G. R, High-sensitivity separations of sodium dodecyl sulfate-protein complexes with capillary gel electrophoresis, LC-GC, 17(2), 132, 1999. 

Lin et al. [95] used capillary electrophoresis with dual cyclodextrin systems for the enantiomer separation of miconazole. A cyclodextrin-modified micellar capillary electrophoretic method was developed using mixture of /i-cyclodextrins and mono-3-0-phenylcarbamoyl-/j-cyclodextrin as chiral additives for the chiral separation of miconazole with the dual cyclodextrins systems. The enantiomers were resolved using a running buffer of 50 mmol/L borate pH 9.5 containing 15 mmol/L jS-cyclodextrin and 15 mmol/L mono-3-<9-phcnylcarbamoyl-/j-cyclodextrin containing 50 mmol/L sodium dodecyl sulfate and 1 mol/L urea. A study of the respective influence of the /i-cyclodcxtrin and the mono-3-(9-phenylcarbamoyl-/i-cyclodextrin concentration was performed to determine the optical conditions with respect to the resolution. Good repeatability of the method was obtained. 

Micellar electrokinetic capillary chromatography (MECC), in contrast to capillary electrophoresis (CE) and capillary zone electrophoresis (CZE), is useful for the separation of neutral and partially charged species [266,267]. In MECC, a surfactant, usually sodium dodecyl sulfate (SDS), is added to the buffer solution above its critical micellar concentration to form micelles. Although SDS is certainly the most popular anionic surfactant in MECC, other surfactants such as bile salts have proved to be very effective in separating nonpolar analytes that could not be resolved using SDS [268]. 

Capillary electrophoresis has been applied by Chen and Gu (281) for simultaneous determination of oxytetracycline, tetracycline, chlortetracycline, and doxycycline residues in bovine milk. Separation was performed on a noncoated capillary column, 57 cm total length with 50 cm effective length, 75 m internal diameter (i.d.) and 375 m outside diameter (o.d.), using a mobile phase containing 10 mM sodium dodecyl sulfate, 50 mM borate, and 50 mM phosphate, pH 8.5. Under these conditions, concentrations below 10 ppb could be determined in milk using an ultraviolet spectrophotometer set at 370 nm. 

Chang and Lin [50] determined vigabatrin in human plasma by capillary electrophoresis and laser-induced fluorescence after precolumn derivatization with 5-carboxytetramethylrhodamine succinimidyl ester. Optimal separation and detection were obtained with an electrophone buffer of 50 mM sodium borate (pH 9.5) containing 10 mM sodium dodecyl sulfate and a green He-Ne laser with fluorescence detection at 589 nm and excitation at 543 nm. The assay was rectilinear over the concentration range of 1.5-200 /iM and the lower limit of detection was 0.13 /iM. Both the... 

K Tsuji. Evaluation of sodium dodecyl sulfate non-acrylamide polymer gel-filled capillary electrophoresis for molecular size separation of recombinant bovine somatotropin. J Chromatogr 652 139-147, 1993. 

Karim MR, Janson JC, Takagi T. Size-dependent separation of proteins in the presence of sodium dodecyl sulfate and dextran in capillary electrophoresis effect of molecular weight of dextran. Electrophoresis 1994 15 1531-4. 

Manabe, T., Oota, H., and Mukai, J. Size separation of sodium dodecyl sulfate complexes of human plasma proteins by capillary electrophoresis employing linear polyacrylamide as a sieving polymer. Electrophoresis, 19, 2308, 1998. 

J.M. Herrero-Martlnez, E.F. Simo-Alfonso and G. Ramis-Ramos, Separation of homologues and isomers of linear aUcylbenzenesulfonates by capillary electrophoresis with sodium dodecyl sulfate, carboxylic acids and bile salts, Electrophoresis, 24, 681-686, 2003. 

Table 3.2 summarizes the most popular proteome profiling techniques. Recent developments introduced capillary electrophoresis [92] not only for DNA analysis and sequencing, but also for high-resolution protein separation, based on their size (capillary electrophoresis-sodium dodecyl sulfate [CE-SDS]) and capillary isoelectric focusing (cIEF) point... 

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