Sodium dihydrogen orthophosphate

Materials Required Plasma protein solution 2.0 ml mixed phosphate buffer pH 7.0 with azide [To 1000 ml of a solution containing 1.8% w/v of disodium hydrogen orthophosphate and 2.3% w/v of sodium chloride and sufficient of a solution containing 0.78% w/v of sodium dihydrogen orthophosphate and 2.3% w/v of sodium chloride (about 280 ml) to produce a pH of 7.0 Dissolve sufficient sodium azide in the resulting solution to give a 0.02% w/v solution] 1000 ml ... 

Sodium dihydrogen orthophosphate (2H2O) [7558-80-7] M 156.0. Crystd from warm water (0.5ml/g) by chilling. 

The chromatographic procedure may be carried out using (a) two stainless steel columns in series the first (25 cm x 4.6 mm) packed with particles of silica, the surface of which has been modified with chemically bonded hexysilyl groups (5 /im) (Spherisorb C6 is suitable) and the second (25 cm x 4.6 mm) packed with cation exchange resin (10 /an) (Partisil-10 SCX is suitable) (b) as the mobile phase at a flow rate of 1.0 ml/min, a mixture of 25 volumes of acetonitrile, 25 volumes of a phosphate buffer solution prepared by dissolving 58.5 g of sodium dihydrogen orthophosphate... 

Disodium hydrogen phthalate-sodium dihydrogen orthophosphate... 

Disodium hydrogen phthalate-sodium dihydrogen orthophosphate Dipotassium hydrogen phthalate-potassium dihydrogen orthophosphate Potassium dihydrogen orthophosphate-sodium hydroxide Borate buffer, phosphate buffer... 

Phosphate buffered saline (PBS) 8.5 g of sodium chloride, 1.07 g of disodium hydrogen orthophosphate (or 2.7 g of NagHP04 2H20), 0.39 g of sodium dihydrogen orthophosphate (or 0.51 g of NaH2P04 2H20). Make up to 1 L with disdlled water. 

Acid sodium phosphate E339 Kalipol 32 monosodium orthophosphate monosodium phosphate phosphoric acid, monosodium salt primary sodium phosphate sodium biphosphate sodium dihydrogen orthophosphate sodium dihydrogen phosphate. 

Phosphate-buffered saline (PBS) 120 mM sodium chloride, 11.5 mM Sodium dihydrogen orthophosphate, 31.3 mM potassium dihydrogen orthophosphate, pH 7.4-7.6. 

Crystals of sodium meta-arsenate-phosphates with P/As ratios of 1, 2, 3, and 4 are prepared by the following procedure (57,60). A mixture of calculated amounts of sodium dihydrogen orthophosphate and sodium dihydrogen orthoarsenate is melted at 620°C. and tempered at 470°C. for 24 hr. The resulting product is hard, needle-shaped crystals. When a mixture of the phosphate and the arsenate with a P/As ratio of 5 is treated by a similar procedure, needle-shaped crystals with considerable amounts of sodium trimetaphosphate are produced. 

Sodium triphosphate I, the stable phase, is produced when an intimate mixture of 1 mol of sodium dihydrogen orthophosphate with 2 mols of disodium hydrogen orthophosphate is heated to 540 to 580 for 2 hours in a platinum dish. ... 

Sodium trimetaphosphate can be prepared by (1) heating sodium dihydrogen orthophosphate for 5 hours at 530° or (2) tempering a glassy sodium metaphosphate at 520° fo 12 hours. If the product obtained by the first of these procedures is not entirely water-soluble, additional heating will be necessary to eliminate the insoluble polymer that forms at a lower temperature. 

If sodium dihydrogen orthophosphate is heated below 240°C, only the acid pyrophosphate, Na2H2P207, is produced. On heating above 240°C, this salt may be changed to a long-chain material known as Maddrell salt [44] (two polymorphic forms), or to sodium trimetaphosphate, NajPjOg. These two compounds are typical of the variety of polyphosphates and metaphosphates which exist... 

Sodium trimetaphosphate (sodium cyclotriphosphate), NajPjOg, can conveniently be prepared by heating and cooling sodium dihydrogen orthophosphate under the correct conditions, but the action of heat on this latter compound can in fact give rise to many products (5.160). The complexities of the sodium metaphosphate system are indicated in Figure 5.20. 

HPLC-ED analysis of nicotine (Figure 6.61) in dog plasma (0.1 mL) after protein precipitation with acetonitrile has been described." An ODS-modified silica column was used with (acetonitrile-methanol, 3 + l)-aq. sodium dihydrogen orthophosphate (2 mmol L ) containing sodium octyl sulfate (0.25 mmol L ) (1 + 19) (final pH 3). Detection was at PGEs (Ei +0.45 V, E2 +0.75 V V5 Pd). A LoD of 20pgL was... 

X Phosphate Standards Sodium dihydrogen orthophosphate monohydrate (NaH2P04.H20) (BDH AnalaR Merck Chemicals, Darmstadt, Germany) is dried in an oven at 110° for 3 h or overnight to remove water prior to making up standard solutions. Prepare a 1 mM stock solution and dilute it in water to obtain seven 5 x standard concentrations (500, 250,150,100, 50, 25, and 5 iiM 5x stocks). Store them in multiple aliquots (e.g., 100 /xl each) and indefinitely freeze in well-sealed tubes. 

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