Plasma protein solutions

Materials Required Plasma protein solution 2.0 ml mixed phosphate buffer pH 7.0 with azide [To 1000 ml of a solution containing 1.8% w/v of disodium hydrogen orthophosphate and 2.3% w/v of sodium chloride and sufficient of a solution containing 0.78% w/v of sodium dihydrogen orthophosphate and 2.3% w/v of sodium chloride (about 280 ml) to produce a pH of 7.0 Dissolve sufficient sodium azide in the resulting solution to give a 0.02% w/v solution] 1000 ml ... 

Size Exclusion Chromatography (Chapter 31) has also been included as a means of analysis for substances that undergo separation more or less as per their molecular size, viz., insulin and human insulin—for proteins of higher molecular weight corticotrophin—for impurities of higher molecular weights and plasma-protein solution—for polymers and aggregates. 

AES is used in pharmacopoeial assays of (1) Na in albumin solution and plasma protein solution (2) K, Na and barium (Ba) in calcium acetate used to prepare dialysis solutions (3) Ca in adsorbed vaccines (e.g. diphtheria and tetanus). 

Kaplan [66] investigated the persistence of VACV and determined inactivation curves at temperature ranges between 50°C-60°C. The results from this study demonstrated a rapid fall of infectivity followed by a complete inactivation at a much slower rate. VACV can be inactivated by pasteurization at 60°C in a variety of protein solutions [90], Approximately a 2 logi0 inactivation of VACV was observed after 1 h pasteurization in alphai-proteinase inhibitor solution within 3 h of exposure, no active VACV was detectable. Similarly, in human plasma protein solution, VACV was reduced by 4 logio within 30 min, and by 2 h vims activity was undetectable. 

Nl. Nitschmann, H., Kistler, F., Renfer, H., HSssig, A., and Joss, A., A heat stable human plasma protein solution obtained by desalting. Vox Sanguinis 1, 183 (1956). 

Physical methods cryopredpitation is often used as the initial step for the production of Factor VIII and fibrinogen. It has no impact on the viral safety of such products. Subsequent purification techniques, such as precipitation by agents other than ethanol or chromatographic separation as well as procedures for viral inactivation are used to obtain the finished products. Cryoprecipitate-depleted plasma can be used for the separation of other coagulation factors or plasma protein solutions. 

We have shown that the morphology of the adsorbed individual molecule and the protein film is dependent upon the surface to which the protein is adsorbed and the wall shear rate at which the protein solution contacts the surface. We have studied the interaction of proteins with surfaces from two approaches the first involved observations of the tertiary structures of individual molecules, and the second involved the use of a flow cell and the exposure of test materials to flowing, dilute, purified human plasma protein solutions. On the hydrophobic surfaces of polystyrene and polycarbonate, albumin and fibrinogen films form networks. On the surface of the hard segment model polyurethane, the morphology of the adsorbed film is very similar to that formed on the hydrophobic polystyrene and polycarbonate surfaces. On the peu-2000, the lowest... 

Acute hypotension has been seen in a few patients taking enalapril when they were given a rapid infusion of albumin-containing stable plasma protein solution (SPPS). Another case occurred in an infant taking captopril when given albumin 4%l A few other cases have been described with gelatin-type colloids in patients taking ACE inhibitors (cilazapril, enalapril, lisinopril). 

Young K. H3 otension from die interaction of ACE inhibitors widi stable plasma protein solution. Anaesthesia (1993) 48, 356. 

Harrison GA, Torda TA, Schiff P (1971) Hypotensive effects of stable plasma protein solutions (SPPS) a preliminary communication. Med J Aust 2 1308-1309 Hartmann G (1967) Fettemulsionen (Intralipid) in der inneren Medizin. Wien Med Wo-chenschr 177 51-55... 

Isbister JP, Biggs JC (1976) Reactions to rapid infusion of stable plasma protein solution during large volume plasma exchange. Anaesth Intensive Care 4 105-107 Ishizaka K (1963) Gammaglobulin and molecular mechanisms in hypersensitivity reactions. Prog Allergy 7 32-106... 

Holden AJ, Littlejohn D, Fell GS (1996) Determination of citrate in plasma protein solutions by UV-visible spectrophotometry and ion chromatography. J Pharm Biomed Anal 14 713-719... 

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