Disodium hydrogen orthophosphate

Materials Required Plasma protein solution 2.0 ml mixed phosphate buffer pH 7.0 with azide [To 1000 ml of a solution containing 1.8% w/v of disodium hydrogen orthophosphate and 2.3% w/v of sodium chloride and sufficient of a solution containing 0.78% w/v of sodium dihydrogen orthophosphate and 2.3% w/v of sodium chloride (about 280 ml) to produce a pH of 7.0 Dissolve sufficient sodium azide in the resulting solution to give a 0.02% w/v solution] 1000 ml ... 

Disodium hydrogen orthophosphate (anhydrous) [7558-79-4] M 142.0. Crystd twice from warm water, by cooling. Air dried, then oven dried overnight at 130 . Hygroscopic, should be dried before use. 

Trichloroacetic acid (TCA [81.6 g]) and disodium hydrogen orthophosphate dodecahydrate Na2HP04.12H20 (89.6 g) are dissolved in 600 ml water. Paraquat dichloride (l,l-dimethyl-4,4 -bipyridinium dichloride) (70 ml 36%) is then added, and the solution is adjusted to 1,000 ml with distilled water. The mixture is then cooled on ice to prevent formation of large crystals that are slow to dissolve on thawing and is stored at -15 °C. Extractant A is prepared from 5 ml distilled water adjusted to 1,000 ml with stock solution. Extractant B is prepared by adding 10 ml stock solution (0.1 mM ATP) to approximately 950 ml Extractant A and adjusting to 1 1 with further Extractant A. Normally it is advisable to prepare 51 of stock solution so that the same batch of extractant is used for the complete analysis. 

J This compound is a colloidal hydroxyapatite, containing about 5% water, which is formed by slow precipitation from calcium chloride and disodium hydrogen orthophosphate solutions at 100° and pH of 9 to 10. 

Disodium hydrogen orthophosphate, Na2HP04, is obtained in the form of dodecahydrate by neutralizing phosphoric acid with sodium carbonate. It is the ordinary sodium phosphate of commerce, and is a white,... 

Sodium pyrophosphate, Na4P207.—The pyrophosphate is obtained in its anhydrous form by heating disodium hydrogen orthophosphate ... 

Phosphate buffered saline (PBS) 8.5 g of sodium chloride, 1.07 g of disodium hydrogen orthophosphate (or 2.7 g of NagHP04 2H20), 0.39 g of sodium dihydrogen orthophosphate (or 0.51 g of NaH2P04 2H20). Make up to 1 L with disdlled water. 

Sample preparation Condition a Sep-Pak C18 SPE cartridge with 5 mL MeOH then 5 mL water. 1 mL Urine + 100 pL 0.5 M tetrabutylammonium bromide in water, vortex 30 s, add to SPE cartridge, elute with 9 mL MeCN buffer 3 97, make up to 10 mL with mobile phase, inject tui aliquot. (Buffer was 100 mL 0.5 M disodium hydrogen orthophosphate + 350 mL water adjusted to pH 4.85 with 1 M citric acid then made up to 500 mL.)... 

The reaction of an equimolar mixture of sodium hydrogen sulfate and disodium hydrogen orthophosphate has been investigated by Poni and Cern tescu (47), Audrieth et al. (48), and Ohashi and Ikeda (40). 

A. The Reaction of Sodium Hydrogen Sulfate with Disodium Hydrogen Orthophosphate... 

Disodium hydrogen orthophosphate Disodium hydrogen phosphate. See Sodium phosphate dibasic anhydrous... 

Sodium triphosphate I, the stable phase, is produced when an intimate mixture of 1 mol of sodium dihydrogen orthophosphate with 2 mols of disodium hydrogen orthophosphate is heated to 540 to 580 for 2 hours in a platinum dish. ... 

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