Smith degradation, of carbohydrates

An example of the use of Smith degradation for carbohydrate sequencing is a study on the sites of AcNeu-Gal-GlcNAc linkages to... 

I. K. Baird, M. I. Holroyde, and D. C. Ellwood, Analysis of the products of Smith degradation of polysaccharides by g.l.c. of the acetylated, derived aldononitries and alditols, Carbohydr. 

L. A. Fransson and I. Carlstedt, Alkaline and Smith degradation of oxidized dermatan sul-phate-chondroitin sulphate copolymers, Carbohydr. Res., 36 (1974) 349-358. 

Albano, R.M., Mauro, S.G., Pavao, M.S.G., and Mourao, P.A.S. (1990) Structural studies of a sulfated L-galactan from Styela plicata (tunicate) analysis of the Smith-degraded polysaccharide. Carbohydr. Res., 208, 163-174. 

Smith and coworkers152 published a relatively complete paper on the thin-layer chromatography, on silica gel, of carbohydrates of low molecular weight. Bancher and coworkers153 reported the thin-layer chromatography of degradation products of carbohydrates, including aldonic acids and aldonolactones. 

The carbohydrate-binding specificity of purified Dolichos biflorus lectin has been studied in detail by Etzler and Kabat.108 In precipitin studies conducted on the Dolichos lectin, 87-99% was precipitated by ovarian cyst A substance, whereas only 60-70% was precipitated by hog A substance the latter result was attributed to the formation of soluble, lectin-oligosaccharide complexes. At substances were more reactive than A2 substances however, no reaction was observed with B or O substances, even following mild hydrolysis with acid or two stepwise, Smith degradations. N-Deacetylation of A substance with... 

In order to gain information into the fine structure of carbohydrate molecules, selective chemical and enzymatic degradations were performed. A useful chemical degradation used in these studies was the Smith degradation, where... 

S. Hizukuri and S. Osaka, A rapid Smith-degradation for the determination of non-reducing, terminal residues of (l->4)-a-D-glucans, Carbohydr. Res., 63 (1978) 261-264. 

T. Krusius and J. Fiime, Use of the Smith degradation in the study of the branching pattern in the complex-type carbohydrate units of glycoproteins, Carbohydr. Res., 90 (1981) 203-214. 

Goldstein IJ, Hay GW, Lewis BA, Smith F. Controlled degradation of polysaccharides by periodate oxidation, reduction, and hydrolysis. Methods Carbohydr Chem 1965 5 361-370. 

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