Skin Tumorigenesis in Mice

Henderson, C., et al., Increased skin tumorigenesis in mice lacking pi class glutathione s-transferases, Proc. Natl. Acad. Sci., 95, 5275, 1998. 

Wallcave L, Garcia H, Feldman R, et al. 1971. Skin tumorigenesis in mice by petroleum asphalts and coal-tar pitches of known polynuclear aromatic hydrocarbon content. Toxicol Appl Pharmacol 18 41-52. 

Sancheti, G. and Goyal, P.K. 2006. Effect of Rosmarinus officinalis in modulating 7,12— dimethylbenz(a)anthracene induced skin tumorigenesis in mice. Phytotherapy Research, 20, 981-986. 

DMBA-initiated mice inhibited the mirex-induced number of tumors per mouse by 63%. DBM also strongly inhibited UV-induced skin sunburn lesions and formation of skin tumors in SKH-1 mice. Topical application of 10 pmol DBM to SKH-1 mice previously initiated with DMBA at 10 min prior to each UV (30 mJ/cm ) irradiation twice a week for 34 weeks inhibited the number of skin tumors per mouse by 91%. Percent of mice with skin tumors was reduced by 44%. In complete UV skin tumorigenic model, topical application of 10 pmol DBM at 10 min before each UV (30 mJ/cm ) irradiation twice weekly for 34 weeks inhibited the number of skin tumors per mouse by 96%, and percent of mice with tumors was decreased by 48%. Our results indicated that DBM strongly inhibited bofli chemical- and UV-induced skin inflammation, sunburn lesions and skin tumorigenesis in mice. 

Iversen OH. 1988. Formaldehyde and skin tumorigenesis in Senear mice. Environ Int 14 23-28. 

Gibbs, N.K., Young, A.R., and Magnus, I.A., Failure of UVR dose reciprocity for skin tumorigenesis in hairless mice treated with 8-methoxypsoralen, Photochem. Pho-tobiol., 42(1), 39 12, 1985. 

Klein, M. Inhibition of skin tumorigenesis in Strain B6AF1/J female mice with maleic anhydride J. Natl. Cancer Inst. 34 (1965) 175-183. 

Xu C, Huang MT, Shen G, Yuan X, Lin W, Khor TO, Conney AH, Tony Kong AN (2006) Inhibition of 7, 12-dimethylbenz(a) anthracene-induced skin tumorigenesis in C57BL/6 mice by sulforaphane is mediated by nuclear factor E2-related factor 2. Cancer Res 66 8293-8296... 

Dibenzoylmethane (DBM) is a minor constituent of licorice and an analogue of curcumin. We previously reported that feeding 1% DBM in the diet strongly inhibited 7,12-dimethylbenzyla[a]anthracene (DMBA)-induced mammary tumorigenesis and formation of leukemias/lymphomas in Senear mice). In this report, we show that topical application of DBM to the backs of mice inhibited chemical- and ultraviolet light (UV)-induced inflammation, sunburn lesions, and formation of skin tumors in mice. Topical application of DBM inhibited TPA-induced edema of mouse ears and skin tumor promotion in CD-I mice in a dose-dependent manner. Topical application of 3 - 10 pmol DBM with 5 nmol TPA twice weekly for 16 weeks in CD-I mice previously treated with DMBA inhibited the number of skin tumors per mouse by 65 - 93%, and percent of mice with tumors was inhibited by 29 - 50%.. Topical application of 10 pmol DBM with mirex (a non-TP A type tumor promoter) 3 times a week for 18 weeks in... 

In 1977, Levin etal. [49] studied the carcinogenic toxicities of trans-7,8-dihydroxy-7,8-dihydrobenzo(a)pyrene (BP-7,8-dihydrodiol) on mouse skin via a two-stage tumorigenesis system. The applied doses of this toxic hydrocarbon, on the backs of CD-I mice, were 50, 100 and 200 nmoles of the (-h)- and (—)-enantiomers separately. Tumour formation on the skin of the mice was observed after several weeks. The results of the tumour formation due to the application of this hydrocarbon are summarized in Table 4.5. The effects of the dose of BP-7,8-dihydrodiol on the percentage of mice with papilloma formation and the number of papillomas per mouse are shown in Figures 4.6(a) and 4.6(b), respectively. It may be concluded from this table that the (—)-enantiomer is more toxic than the (-l-)-enantiomers... 

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