Single-stranded oligonucleotides

Moreover, disposable electrochemical sensors for the detection of a specific sequence of DNA were realised by immobilising synthetic single-stranded oligonucleotides onto a graphite or a gold screen-printed electrode. Tire probes became hybridised with different concentrations of complementary sequences present in the sample. 

Palchetti I, Mascini M (2005) Electrochemical Adsorption Technique for Immobilization of Single-Stranded Oligonucleotides onto Carbon Screen-Printed Electrodes. 261 27-43 Pascal R, Boiteau L, Commeyras A (2005) From the Prebiotic Synthesis of a-Amino Acids Towards a Primitive Translation Apparatus for the Synthesis of Peptides. 259 69-122 Paulo A, see Santos I (2005) 252 45-84 Perez EM, see Leigh DA (2006) 265 185-208 Perret F, see Coleman AW (2007) 277 31-88 Perron H, see Coleman AW (2007) 277 31-88 Pianowski Z, see Winssinger N (2007) 278 311-342 Piestert F, see Gansauer A (2007) 279 25-52... 

Mann and coworkers proposed a unique method to prepare nanohybrids of mesoporous silica and gold nanoparticles mediated by programmed DNA hybridization, as illustrated in Figure 4.21 [186], MCM-41 nanoparticles were first functionalized with a 3 -amino-terminated 12-base single strand oligonucleotide... 

Preliminary results of DNA sequence detection using the OFRR are thoroughly described by Suter, et al.32 The experimental setup described above for tracking the resonant mode shift is used. An OFRR with RI sensitivity of about 7 nm/RIU was produced and used for these experiments. The OFRR surface was functionalized with 3APS and then DMA was used as an amine-amine crosslinker. The single-stranded oligonucleotide capture probe was synthesized with an amine functional group connected to the 5 end by a 6-carbon linker, and has a 25 base-pair sequence. ... 

Electrochemical Adsorption Technique for Immobilization of Single-Stranded Oligonucleotides onto Carbon Screen-Printed Electrodes I. Palchetti M. Mascini... 

Reactions involving T(—H) in dinucleotides and single-stranded oligonucleotides led to the formation of tandem lesions in deaerated aqueous solution involving covalent bond formation between the 5-methyl and C-8 of The d(G T)... 

According to the available literature, five fluorine-18-labelling reagents have been prepared and used for prosthetic conjugation with a single-stranded oligonucleotide [14,188,189,277-281]. 

Fig. 10. Fluorine-18-labelled reagents for coupling to single-stranded oligonucleotides.

A/-[3-(2-[ F]fluoropyridin-3-yloxy)-propyl]-2-bromoacetamide (p F]FPyBrA) was recently prepared by nucleophilic / eferoaromatic radiofluorination using a three-step radiochemical pathway and obtained in 20% overall non-decay-corrected yield in less than 85 min. In this reagent, the pyridinyl moiety carries the radioactive fluorine and the 2-bromoacetamide function ensures the alkylation of phosphorothioate monoester groups at the 3 - or 5 -end of a single-stranded oligonucleotide [18],... 

---