Oligonucleotides single-strand

Phosphorothioate Oligonucleotides Single-stranded phosphorothioate ODNs (PS ODN), either immunostimulatory or ASO, administered parenter-ally appear rapidly in plasma where they are bound to hydrophilic sites on... 

Conformational analysis of oligonucleotide single strands containing 2, 3 -dideoxygluco-pyranosyl building blocks (162), (the so-called homo-DNA) has been used to address the question as to why nature chose pentose, rather than hexose sugar units for the construction of 128... 

Krull et al. [50] reported the ion currents through s-BLMs on Ag wire that were used to monitor hybridization of 20 mer and 25 mer oligonucleotides (single-stranded DNA oligonucleotides that were modified by attachment of a 16-carbon aliphatic chain). The results indicated that hybridization could be detected for mixed-base sequences, and for partially complementary sequences. Quantitative results are dependent on the degree of surface occupancy by DNA, on the degree of complementarity of sequences, and on the base sequence within the oligonucleotides. 

The activity assays were performed under the same conditions with the two proteins. DNA binding was performed by gel shift assay as described in the text, on oligonucleotide single-stranded (ss) DNA or double-stranded (ds) DNA, these substrates containing the left part or the right part of the nicking site, nd. Not determined. 

Eschenmoser, A. and Dobler, M. (1992) Why Pentose and Not Hexose Nucleic Acids Part I. Intnxluction to the Problem, Conformational Analysis of Oligonucleotide Single Strands Containing 2, 3 -Dideoxyglucopyianosyl Building Blocks (Homo-DNA ), and Reflections on the Conformation of A- and B-DNA, Helv. Chim. Acta 75, 218-259. 

In the sense strategy, the oligonucleotide (single-stranded or double-stranded) binds and traps a protein involved in the control of gene... 

Fig. 9. Mutagenesis by a synthetic oligonucleotide of a cloned sequence available in single-stranded form (a) single-stranded M13 template containing uracil (U) residues (b) double-stranded product, uracil residues are not mutagenic (c) strong selection for M13 phages containing mutation of interest (23).

The oligonucleotide population is then hybridized to single-stranded DNA and primed synthesis is carried out as described above. 

Moreover, disposable electrochemical sensors for the detection of a specific sequence of DNA were realised by immobilising synthetic single-stranded oligonucleotides onto a graphite or a gold screen-printed electrode. Tire probes became hybridised with different concentrations of complementary sequences present in the sample. 

The double-stranded DNA to be amplified is heated in the presence of Taq polymerase, Mg2+ ion. the four deoxynucleotide triphosphate monomers (dNTPs), and a large excess of two short oligonucleotide primers of about 20 bases each. Each primer is complementary to the sequence at the end of one of the target DNA segments. At a temperature of 95 °C, double-stranded DNA denatures, spontaneously breaking apart into two single strands. 

Antisense therapy means the selective, sequence-specific inhibition of gene expression by single-stranded DNA oligonucleotides. By hybridizing to the target mRNA, which results in a subsequent double-helix formation, gene expression is blocked. This process can occur at any point between the conclusion of transcription and initiation of translation or even possibly during translation. 

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