Silica affinity chromatography

J. D. Protein-doped monolithic silica columns for capillary liquid chromatography prepared by the sol-gel method applications to frontal affinity chromatography. Anal Chem... 

Glad, M., Ohlson, S., Hansson, L., Mansson, M.-O., and Mosbach, K., High-performance liquid affinity chromatography of nucleosides, nucleotides and carbohydrates with boronic acid-substituted microparticulate silica, J. Chromatogr., 200, 254-260, 1980. 

Porous supports like agarose, pol3mrethacrylate, or silica beads are generally used in current applications of affinity chromatography. However, in the past several years other types of supports have also become available commercially. Many of these newer materials have properties that give them superior performance in certain applications. Materials that fall in this category include nonporous supports, membranes, flow-through beads, continuous beds and expanded-bed particles. 

As discussed earlier, the proteins used as chiral selectors in affinity chromatography cannot be used under the high-pressure HPLC with a variety of mobile phases therefore, these proteins were immobilized with some solid support such as hydroxyethylmethacrylate, polystyrene-divinylbenzene, polyethylene fibers, and silica gel [14,15]. Avariety of techniques have been used for the immobiliza-... 

Figueroa, A., Corradini, C., Feibush, B., and Karger, B., High-performance immobilized-metal affinity chromatography of proteins on iminodiacetic acid silica-based bonded phases, J. Chromatogr., 371, 335, 1986. 

Support materials for low-pressure affinity chromatography include agarose (cross-linked with epichlorohydrin), cellulose, dextran, silica, and polyacrylamide 62 in HPLC a rigid, highly porous, hydrophilic polymer is typically used. Large pore sizes are necessary, as either the analyte or the affinity ligand are macromolecules. To provide unhindered access of... 

The ultimate combination of HPLC and AC is effectuated in High Performance Affinity Chromatography (HPAC).47 The development of this hybrid technique was highly assisted by the use of modified silica. Traditional polysaccharide supports may not be used for HPAC, because they lack mechanical stability to withstand the high pressure drops, inherent to this method. Modified silica beads are well suited. These may be coated with active groups as in normal AC applications. Additionally, if the separation requires the use of an organic stationary phase, the silica beads are modified with a silane or polymer with subsequent deposition of polysaccharides such as dextrans, agarose or cellulose.50... 

Heparinlike sulfated dextran derivatives, like carboxymethyldextran benzylamide sulfonates (CMDBS), have been immobilized on silica beads. By high-performance liquid affinity chromatography (HPLAC), they allow a good recovery of thrombin, with a yield of 80%. The affinity constant was estimated K lO M ) and was found superior to the value obtained between thrombin and heparin. On the contrary, the affinity of dextran derivatives for ATIII is estimated at a lower value than that of heparin [17],... 

H. Lakhiari, J. Jozefonvicz, and D. Muller, Separation and purification of insulins on coated silica support functionalized with sialic acid by affinity chromatography, J. Liquid Chromatogr. Related Technol. 19 2423 (1996). 

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