Affinity chromatography porous silica

Porous supports like agarose, pol3mrethacrylate, or silica beads are generally used in current applications of affinity chromatography. However, in the past several years other types of supports have also become available commercially. Many of these newer materials have properties that give them superior performance in certain applications. Materials that fall in this category include nonporous supports, membranes, flow-through beads, continuous beds and expanded-bed particles. 

Support materials for low-pressure affinity chromatography include agarose (cross-linked with epichlorohydrin), cellulose, dextran, silica, and polyacrylamide 62 in HPLC a rigid, highly porous, hydrophilic polymer is typically used. Large pore sizes are necessary, as either the analyte or the affinity ligand are macromolecules. To provide unhindered access of... 

Tayot J-L, Tardy M (1980) Isolation of cholera toxin by affinity chromatography on porous silica beads with covalently coupled ganglioside Gmi- In Adv. Exp. Med. Biol. 125 471 -478. 

Adsorption chromatography exploits differences in the relative affinity of solutes for a solid adsorbent used as the stationary phase. Common stationary phase materials for adsorption chromatography are porous silica gel, activated alumina, activated carbon, magnesium oxide, carbonates, and highly cross-linked polymers such as styrene-divinylbenzene and methac-rylates. The chemical natures of these adsorbent stationary phase materials make them well suited for separations of solute mixtures that differ in polarity and chemical functionality. For example, silica is an acidic adsorbent that retains basic compounds to a greater extent than nonbasic ones. In contrast, alumina... 

Porous silica is one of the ideal rigid supports, which can be made with different pore size distribution to efficiently increase the macromolecular transfer inside. However, it is difficult to be used directly for affinity chromatography because of its complex nonspecific adsorption of molecular target by hydrophobic interaction. Also, its poor stability at basic conditions limits its application. 

B. Anspach, K.K. Unger, J. Davies, and M.T.W. Heam Affinity chromatography with triazine dyes immobilized onto activated non-porous monodisperse silicas J. Chromatogr. A, 457... 

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