Signal components

GPI anchoring is a posttranslational modification occurring in the endoplasmic reticulum where preassembled GPI anchor precursors are transferred to proteins bearing a C-terminal GPI signal sequence. The GPI anchor precursors are synthesized in the endoplasmic reticulum by sequential addition of sugar and other components to phosphatidylinositol. Protein GPI anchors are ubiquitous in eukaryotic cells. In mammalian cells, GPI anchored proteins are often found in lipid rafts which are subdomains of the plasma membrane, containing various signaling components. 

FT of harmonic signals yields essentially two types of information First the main signal component, i.e., the peak in the FT spectrum that corresponds to the applied frequency (hereafter... 

These techniques offer even more interesting noise-filtering properties as they are capable of localizing time- and frequency-varying noise and signal components. 

Cell lysis When studying signaling components, it is important that the integrity and phosphorylation states of proteins of interest are not altered during cell lysis and the subsequent preparation of the samples prior to analysis. The principal precautions include the use of inhibitors of protein phosphatases and of proteases, in addition to working speedily and keeping samples cold (0 to 4°). 

Alberts, A. C. and Werner, D. I. (1993) Chemical recognition of unfamiliar conspecifics by green iguanas functional significance of different signals components. Anim. Behav. 46, 197-199. 

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