Short-interfering RNA

Screening. See High-throughput screening Second messenger systems calcium ion, 83 description of, 24 production of, 25f Series hyperbolae, 38 Serotonin, 150, 151 f Seven transmembrane receptors, 3-4 Shennong Herbal, 147 Short interfering RNA duplex molecules, 184... 

Bridge AJ, Pebernard S, Ducraux A, Nicoulaz AL, Iggo R (2003) Induction of an interferon response by RNAi vectors in mammalian cells, Nat Genet 34 263-264 BrummeUcamp TR, Bernards R, Agami R (2002) A system for stable expression of short interfering RNAs in mammahan cells. Science 296 550-553... 

Hamasaki K, Nakao K, Matsumoto K, Ichikawa T, Ishikawa H, Eguchi K (2003) Short interfering RNA-directed inhibition of hepatitis B vims replication. EEBS Lett 543 51-54 He ML, Zheng B, Peng Y, Peiris IS, Poon LL, Yuen KY, Lin MC, Kung HP, Guan Y (2003) Inhibition of SARS-associated coronavims infection and replication by RNA interference. JAMA 290 2665-2666... 

Morrissey DV, Blanchard K, Shaw L, Jensen K, Lockridge JA, Dickinson B, McSwiggen JA, Vargeese C, Bowman K, Shaffer CS, Pohsky BA, Zinnen S (2005a) Activity of stabilized short interfering RNA in a mouse model of hepatitis B virus replication. Hepatology 41 1349-1356... 

Raymond, C. K., Roberts, B. S., Garrett-Engele, P., Lim, L. P., and Johnson, J. M. (2005). Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs. RNA 11, 1737-1744. 

RNAi Approach This is the use of short interfering RNA (siRNA, sometimes called small interfering or silencing RNA) to interfere with the expression of a particular gene. The siRNAs are double-stranded RNAs of 20-25 nucleotides. It is envisaged that if the biological pathway of a disease is identified, siRNA could interfere to turn off the activity of the gene involved in the pathway and provide therapeutic effect. 

Semizarov D, Frost L, Sarthy A et al (2003) Specificity of short interfering RNA determined through gene expression signatures. Proc Natl Acad Sci USA 100 6347-6352... 

Scacheri PC, Rozenblatt-Rosen O, Caplen NJ et al (2004) Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells. Proc Natl Acad Sci USA 101 1892-1897... 

Hornung V, Guenthner-BiUer M, Bourquin C et al (2005) Sequence-specific potent induction of IFN-alpha by short interfering RNA in... 

HT-RNAi screens of mammalian cells primarily use either short interfering RNA (siRNA) or small-hairpin RNA (shRNA) libraries. Although there are similarities between the two platforms, screening using siRNA libraries has the advantages of having minimal... 

A system for stable expression of short interfering RNAs in mammalian cells. Science 296 550-553... 

Ptasznik A, Nakata Y, Kalota A et al. Short interfering RNA (siRNA) targeting the Lyn kinase induces apoptosis in primary, and drug-resistant, BCR-ABL1(+) leukemia cells. AarMe<72004 10 1187-1189. 

Karagiannis TC, El-Osta A (2005) RNA interference and potential therapeutic applications of short interfering RNAs. Cancer Gene Ther 12 787-795... 

Fritsch, L., Martinez, L.A., Sekhri, R., Naguibneva, I., Gerard, M., Vandromme, M., Schaeffer, L., and Harel-Bellan, A. (2004) Conditional gene knock-down by Cre-dependent short interfering RNAs. EMBO Rep. 5,178-182. 

Fig. 8.9 A multifunctional nanoparticle the cRGD sequence binds to a cell surface, the nanoparticle is absorbed by endocytosis and can be tracked by a fluorescent dye before the particle disintegrates to deliver short interfering RNA (siRNA) sequences [23]...

Tuzmen, S., Kiefer, J., and Mousses, S. (2007) Validation of short interfering RNA knockdowns by quantitative real-time PCR. Methods Mol Biol. 353 177-203. 

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