Interferon response

Bridge AJ, Pebernard S, Ducraux A, Nicoulaz AL, Iggo R (2003) Induction of an interferon response by RNAi vectors in mammalian cells, Nat Genet 34 263-264 BrummeUcamp TR, Bernards R, Agami R (2002) A system for stable expression of short interfering RNAs in mammahan cells. Science 296 550-553... 

Ito X Kanzler H. Duramad O. Cao W, Liu YJ Specialization, kinetics, and repertoire of type 1 interferon responses by human plasmacytoid pre-dendritic cells. Blood 2006 107 2423-2431. 

Ilback N-G, Wesslen L, Pauksen K, Stalhandske T, Friman G, Fohlman J. Effects of the antiviral WIN 54954 and the immune modulator LS 2616 on cachectin/TNF and gamma-interferon responses during viral heart disease. Scand J Infect Disease Suppl 1993 88 117-123. 

Haller O, Kochs G, Weber F. The interferon response circuit induction and suppression by pathogenic viruses. Virology. 2006 344 119-130. 

Some of the challenges in performing efficient transfection are as described in detail in the chapter, to reduce toxicity, achieve higher efficiency without deleterious manipulation of gene expression machinery, reduce lipid-induced off target effects, and interferon responses. The procedure of transfection is still relatively poorly understood and the challenges posed to the researcher are still daunting at times. The objective of this chapter is to equip the user with vital information to enhance the success of transfection experiments. 

Reynolds A, Anderson EM, Vermeulen A, Fedorov Y, Robinson K, Leake D, Kar-pilow J, Marshall WS, Khvorova A. Induction of the interferon response by siRNA is cell type- and duplex length-dependent. RNA 2006 12(6) 988-93. 

Interferon induction in normal and leukemic lymphocyte cultures with tilorone has been observed50. The interferon response observed in normal lymphocyte cultures appeared to be correlated with the toxic effect of tilorone. The effect observed in leukemic cultures required higher concentrations of tilorone, but, similarly, appeared to be related to cell viability. Tilorone has been reported to stimulate production of interferon by itself in mouse embryo fibroblasts and, in combination with poly rl poly rC/DEAE-dextran in mouse L929 cells51. Human foreskin fibroblasts were not stimulated. The degree of synergism between tilorone and the nucleotide-dextran complex was proportional to the concentrations of tilorone and poly rl/poly rC and was influenced by the times of addition of the compounds relative to each other. 

H. A. R Bluyssen, R Muzaffar, R. J. Vliestra, A. C. J. van der Made, S. Leung, G. R Stark, et al. Combinatorial association and abundance of components of interferon-stimulated gene factor 3 dictate the selectivity of interferon responses. Proc Natl Acad Set, USA, 92, 5645—5649, 1995. 

Sioud M. Induction of inflammatory cytokines and interferon responses by double-stranded and single-stranded siRNAs is sequence-dependent and requires endosomal localization. / Mol Biol. 2005 348 1079-1090. 

The relatively greater importance of poly(I) than of poly(C) in the antiviral activity of poly(I) poly(C) has further been demonstrated in experiments in which the two components were added sequentially to cell cultures122-124). Sequential administration of the homopolymers in the order poly(I) followed by poly(C) led to an equal or greater interferon response than obtained after addition of poly(I) poly(C) itself. However, when the homopolymers were added in the order poly(C) -> poly(I) the antiviral activity of the complex was only partially restored. The mechanism by which the order of addition of... 

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