Proteomic Pattern Diagnostics

The importance of the need for full identification of potential protein markers is controversial. Most papers on SELDI-TOE-MS for cancer diagnosis have been omitting the identification of potential markers beyond the determination of their approximate molecular mass. In the opinion of some investigators, one does not need individual identified markers as long as consistent protein profiles can be obtained [20]. In other words, the diagnosis of a disease should he considered as a prediction and should not be concerned about etiology. 

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E.F. Petricoin and L.A. Liotta, SELDI-TOF-based serum proteomic pattern diagnostics for early detection of cancer. Curr. Opin. Biotech. 15, 24-30 (2004). 

Petricoin EF, Rajapaske V, Herman EH, Arekani AM, Ross S, Johann D, Knapton A, Zhang J, Hitt BA, Conrads TP, Veenstra TD, Liotta LA, Sistare FD. Toxicoproteomics Seram proteomic pattern diagnostics for early detection of drag induced cardiac toxicities and cardioprotection. Toxicol Pathol 2004 32 (Suppl l) 122-30. 

Petricoin, E. F. et al. 2004. Toxicoproteomics Serum proteomic pattern diagnostics for early detection of drug-induced cardiac toxicides and cardioprotection. Toxicologic Pathology 32(Suppl. 1) 122-130. 

P2. Petricoin, E. E., Paweletz, C. P., and Liotta, L. A., Clinical applications of proteomics Proteomic pattern diagnostics. J. Mammary Gland Biol. Neoplasia 7, 433 40 (2002). 

One such approach to disease detection is the use of proteomic pattern diagnostics to detect cancer. The first report of using this technique coupled with mass spectrometry was to identify ovarian cancer. More than two-thirds of ovarian cancer is not detected until it is in an advanced disease stage. Early... 

Yu LR, Zhou M, Conrads TP, Veenstra TD. Diagnostic proteomics Serum proteomic patterns for the detection of early stage cancers. Dis Marker 2003 19(4-5) 209-218. 

Diamandis EP. Proteomic patterns in biological fluids Do they represent the future of cancer diagnostics Clin Chem 2003 49 1271-78. 

The Ciphergen system has also been used to determine serum proteomic patterns in other cancers such as prostate cancer (Al, B2), hepatocellular carcinoma (P6), and non-small cell lung cancer (XI). The protein patterns were determined from a combination of data from more than one capture array, thereby increasing the specificity of the diagnostic signature. 

Diamandis, E.P., Re diagnostic potential of serum proteomic patterns in prostate cancer. J. Urol, 171, 1244-1245 (2004). 

Since the introduction of 2D-PAGE in 1975 [4, 5], long before the term proteomics was even invented [reviewed recently in 1, 6], it has evolved to become one of the most powerful techniques for separating proteins and generating protein patterns. It has become widely applied to a variety of biological samples and therefore promises to have a major effect on medical diagnostics. 

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