Serum lactic dehydrogenase

Amador, E. Dorfman, L. E. and Wacker, W. E. C. Serum lactic dehydrogenase activity. An analytical assessment of current assays. Clin. Chem. (1963), , 391-399. 

Cyprinus carpio 0.5 after 6 days, 300% increase in phosphorylase and 200% increase in glucose-6-phosphatase activities in liver increase in sugar level and serum lactic dehydrogenase activity 18... 

Hepati c 1 (significantly increased relative liver weight significantly increased serum lactic dehydrogenases) ... 

Chronic in vivo hemolysis produces serum lactic dehydrogenase elevations in patients with mitral or atrial valve cardiac prosthesis (J2). In a series of 11 such patients these increases ranged from 1.1 to 1.6 times the upper limit of normal (S29). Blood pH is altered in hemolyzcd specimens because carbonic anhydrase is liberated from the erythrocytes and presumably alters the distribution of H2CO3 and NaHCOs (B2). Hemolysis will effect acid phosphatase activity if the substrate is hydrolyzed by erythrocyte acid phosphatase. Thus, hemolysis would be of concern if phenyl phosphate was the substrate, but would have a negligible effect if )8-glycerophosphate, which is not hydrolyzed by red cell acid phosphatase, was used (Bl). 

Inhibitors of lactic dehydrogenase have been reported in commercial preparations of NAD+ and NADH (B4, M6, S28). The concentration of inhibitory substances varied from lot to lot. In a serum lactic dehydrogenase study with NAD+ from 8 sources, activities were found to vary from 145 to 75 units (B4). Inhibitors of lactic dehydrogenase activity have also been observed in dialyzates in uremic patients (W8) and in human urine (G8). The purity of available substrate can also effect enzyme activity. Schwartz and Bodansky observed that, in 6 batches of fructose 6-phosphate, all weighed to a 0.5 mM concentration, the actual concentration varied from 0.13 mAf to 0.55 mM (S14). 

Ruhmann AG, Berliner DL. 1965. Serum lactic dehydrogenase levels in adult beagle dogs with internally deposited radionuclides. Radiation Res 26 287-294. 

SGPT, serum creatine phosphokinase, or serum lactic dehydrogenase were found in rabbits treated dermally with 20 mL isophorone (Dutertre-Catella 1976). No other indices of liver toxicity were examined therefore, the 20 mL dose cannot be considered a NOAEL for liver effects. 

Clinical Biochemical Determinations of the Serum Serum lactic dehydrogenase (LDH) and glutamic-pyruvic transaminase (GPT) activities were measured on fresh, refrigerated serum within U8 h of sacrificing the animal. Lactic dehydrogenase was measured according to the method of Amador, Dorfman, and Wacker (12). Serum GPT activity was measured according to the method of Wroblewski and LaDue (13). 

Both are abundant in skeletal muscle, myocardium, liver, and erythrocytes, so that hemolysis must be avoided, and in serum they may be assayed spectrophotometrically by their conversion of phosphate-buffered pyruvate to lactate (R6, W16) or oxalacetate to malate (S25) at the expense of added NADH2, when the rate of decrease of optical density at 340 m x thus measmes the serum activities of the respective enzymes. Recently, however, the reverse reaction has been found best for serum lactic dehydrogenase assay (A2a). In conventional spectrophotometric units the normal ranges are 100-600 units per ml for lactic dehydrogenase (W16) and 42-195 xmits per ml for malic dehydrogenase (S25) as before, one conventional spectrophotometric unit per ml = 0.48 pmoles/ minute/liter (W13). 

The effects and the safety of a 5-day regimen of G-CSF (n = 9) or GM-CSF (n = 8) have been compared (10). Most patients complained of flu-Uke symptoms in both groups (six and seven respectively), but rash at the injection site was observed only in four patients treated with GM-CSF. In the G-CSF group, there was a fall in platelet count (below 150 x 10 /1) in five patients, raised serum lactic dehydrogenase activity, and raised uric acid concentrations three patients required transient treatment with allopurinol. 

Cassidy W, Reynolds T. Serum lactic dehydrogenase in the differential diagnosis of acute hepatocellular injury. J Clin Gastroenterol 1994 19 118-21. 

C7. Cohen, L Djordjevich, J., and Ormiste, V., Serum lactic dehydrogenase patterns and other... 

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