Serum cell culture medium

Imidazole antimycotics, ketoconazole, clotrimazole, and miconazole are potent inhibitors of various cytochrome P450-isoenzymes that also affect the metabolism of retinoids. They were fust shown to inhibit the metabolism of RA in F9 embryonal carcinoma cells. When tested in vitm liarazole, a potent CYP-inhibitor, suppressed neoplastic transformation and upregulated gap junctional communication in murine and human fibroblasts, which appeared to be due to the presence of retinoids in the serum component of the cell culture medium. Furthermore, liarazole magnified the cancer chemopreventive activity of RA and (3-carotene in these experiments by inhibiting RA-catabolism as demonstrated by absence of a decrease in RA-levels in the culture medium in the presence of liarazole over 48 h, whereas without liarazole 99% of RA was catabolized. In vivo, treatment with liarazole and ketoconazole reduced the accelerated catabolism of retinoids and increased the mean plasma all-irans-RA-concentration in patients with acute promyelocytic leukemia and other cancels. 

In this in vitro system, the presence of serum in cell culture medium is not necessary, but the type of transwell is important (the total amount of H-triglycerides secreted was two-fold higher when using 3 pm versus 1 pm pore size transwells), and oleic acid supplementation is required for the formation and secretion of CMs as well as the transport of 3-carotene through Caco-2 cells. Finally, the presence of Tween 40 does not affect CM synthesis and secretion in this in vitro cell culture system. Thus, CMs secreted by Caco-2 cells were characterized as particles rich in newly synthesized H-triglycerides (90% of total secreted) containing apolipoprotein B (30% of total secreted) and H-phospholipids (20% of total secreted) and with an average diameter of 60 nm. These characteristics are close to those of CMs secreted in vivo by enterocytes. ... 

In rat blood and in the cell culture medium RPMI 1640 (+15% fetal calf serum) oxaliplatin (4) forms the major biotransformation products [Pt(dach)Cl2], [Pt(dach)(H20)Cl]+ (only in plasma ultrafiltrate), and [Pt(dach)(methionine)]+ (91). 

Table 2.3 Activity of endogenous fragments of functional proteins in tumor and normal fibroblasts. The peptides were tested for their impact on the number of cells after 24 h of incubation in serum-deficient culture medium. The active peptides affect the cell number by 25% and more...

Iseed cell from serum-containing culture medium... 

Indirect protein patterning Serum proteins from cell culture medium PDMS, methylated glass, bacterial grade PS Human epithelial cells, human keratinocytes, human neuroblastoma cells, human breast cancer cells, human fibroblasts, human embryonic kidney cells, murine macrophages Stencil guided plasma induced oxidation of hydrophobic surfaces 2009 [93]... 

Serum proteins from cell culture medium PDMS Human liver carcinoma cells Photocatalitc hydrophilization of PDMS (mask lithography) 2009 [163]... 

Collagen, poly-L-lysine/serum proteins from cell culture medium Crosslinked polyacrylic acid/polyacrylamide multilayer films, PEG Coculture of rat adrenal medulla cells and mouse fibroblasts L929 Photopatteming of polyelectrolyte multilayer film 2009 [110]... 

Serum proteins from cell culture medium PEG CHO-K1, MDCK, Balb/3T3, HepG2 UV patterning of photoresponsive 2009 [165]... 

Second seeding serum proteins from cell culture medium Second seeding contact-inhibition in cell cocultures... 

Serum proteins from cell culture medium Polyvinyl alcohol (PVA)... 

Gefitinib, erlotinib, sunitinib and sorafenib 20 pi Human whole blood, serum, plasma and cell culture medium Turbo ion spray-TSQ 5 3.7 to 13.5 1.9 to 12 Honeywell et al. [136]... 

Cell size distribution of HeLa cells grown in serum-free culture medium (Luebberstedt, 2000), presented as (A) frequency and (B) cumulative undersize (y) and oversize (z) distributions. 

Development of serum-free medium has great value for large-scale biopesticide production. The latest formulations are serum-free, such as SF900II (GIBCO /Invitrogen) and EX-CELL (JRH Biosciences). Cell culture medium supplementation using yeast extract (usually from alcoholic fermentation processes), milk, or soy protein concentrates, can also be an alternative to decrease cell culture medium costs (more details can be found in Chapter 5). 

A 37 mm diameter filter in a similar holder can be used on-line to filter up to a litre of serum-free cell culture medium. Millipore s Sterivex is a tangential flow filter which can be used with a repeating syringe or on line to filter up to 1 litre of growth medium and Gelman s Acrocap (Fig. 8.3) is similar. 

Cell culture medium for PBMCs, RPMI1640 (Gibco-BRL) containing 20% fetal calf serum (FCS), 60 pg/mL penicillin, and 100 pg/mL streptomycin. Supple-... 

Wash medium RPMI 1640 cell culture medium (Sigma), 10% (v/v) foetal calf serum (Invitrogen), 50 mA/HEPES buffer, pH 7.0 (Sigma), 2 mMEDTA... 

Cell culture medium, e.g., Dulbecco s modified Eagle s medium (DMEM) supplemented with L-glutamine (2 mV/). 10% fetal calf serum (FCS), and 0.05%... 

On the day of experiment, trypsinize the adherent cell cultures (see Note 1). After washing twice by centrifugation (5 min, 210 x g), resuspend the cells in the exposure serum-free culture medium, e.g., DMEM supplemented with 25 mM HEPES (DMEM-H). Count viable cells and adjust the cell density to a range of... 

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