Serum androstenedione

Serum sex hormones effect. DION (300 mg androstenedione, 150 mg dehydro-epiandrosterone, 540 mg saw palmetto, 300 mg indole-3-carbinol, 625 mg chrysin, 750 mg Tribulus terrestris), administered daily to healthy 30- to 59-year-old men for 28 days, produced no change in serum concentrations of total T and PSA. DION increased the concentrations of serum androstenedione (342%), free T (38%), DHT (71%), and estradiol (103%). Serum high-density lipoprotein (HDL) cholesterol concentrations were reduced by 5 mg/dL in DION (p < 0.05). Increases in serum free T (r = 0.01), androstenedione (r = 0.01), DHT (r = 0.03), or estradiol (r = 0.07) concentrations in DION were not related to age. The ingestion of androstenedione combined with herbal products increased serum-free T concentrations in older men, but herbal products did not prevent the conversion of... 

Age and familial factors in both men and women influence both plasma adrenal androgens (DHEA and DHEA-S). Adrenal androgen secretion starts to rise about the age of 9, peaks at about age 25, and declines after age 30. In the seventh decade of fife, the adrenal androgen concentrations fall to prepubertal concentrations. Thus in the elderly, the circadian variation in plasma DHEA is relatively blunted and dissociated from the circadian variation in cortisol. The adrenal contribution of serum androstenedione declines with age. DHEA and DHEA-S responses to stimulation with exogenous ACTH are... 

High-dose progestins are used as last-line endocrine therapy [223]. They inhibit the adrenal steroid biosynthesis. The decrease of estrogen levels is comparable to that caused by the administration of aromatase inhibitors. In post-menopausal women, the progestin megestrol acetate (MGA) decreases serum plasma level of DEAH, androstenedione and cortisol to less than 10% [223,224]. 

Guo and co-workers [24,25] have spearheaded the development of MS/MS serum steroid profiles. Their most recent report describes profiling in 11 min of 12 steroids in 200 pi serum with minimal work-up, comprising acetonitrile protein precipitation. The steroids analyzed were as follows DHEA sulfate, DHEA, aldosterone, cortisol, corticosterone, 11-deoxycortisol, androstenedione, estradiol, testosterone, 17-hy-droxyprogesterone, progesterone, and 25-hydroxyvitamin D3. Stable-isotope-labeled internal standards were incorporated for each steroid. An API-5000 instrument was used with the APPI source in positive-ion mode, with the exception of aldosterone, which had greater sensitivity in negative-ion mode. Separation was carried out on a C8 column, which allowed more rapid separation than the more commonly utilized C18. The MRM transitions utilized are shown in Table 5.3.1. The lower level of sensitivity was between 1.5 and 10 pg/ml, dependent on the steroid. The authors were exhaustive in addressing issues of accuracy, recovery (90-110%) and reproducibility (< 12.2% for same-day and between-day). 

IL-6 levels (D3). In addition, Straub et al. (S30) reported that increased IL-6 levels were inversely related to serum DHEA levels in elderly humans. They also showed that DHEA and androstenedione inhibit IL-6 production by peripheral blood mononuclear cells in both males and females. 

Feminizing adrenocortical carcinomas are also rare. They result in elevation of plasma DHEA-S, DHEA, androstenedione, estrone, and estradiol concentrations. Serum cortisol concentrations may be normal or elevated in those patients with Cushing s syndrome. Gynecomastia and sexual dysfunction occur in men and precocious pseudopuberty in women. Steroid hormone production fails to decrease normally after treatment with dexamethasone. 

Thomson S, Wallace AM, Cook B, A 1251-radioimmunoassay for measuring androstenedione in serum and in blood-spot samples from neonates. Clin Chem 1989 35 1706-12. 

Wei, J.Q. Wei, J.L. LucareUi, C. Zhou, X.T. Wang, D.Q. Dai, W.J. Li, S. Li, S.M. Liu, R.T. Serum steroid hormonal profiles by reversed-phase liquid chromatography in patients with 17-hydroxylase deficiency and in an affected family, Clin.Chem., 1992, 38, 76-82. [cortisone hydrocortisone deoxy-cortisol androstenedione testosterone progesterone hydroxyprogesterone desoxycorticosterone]... 

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