Sensitivity enhanced, protein quantification

In the following sections, different methods of establishing functional DNA-protein conjugates are discussed, following a short historic overview of IPCR method development. As IPCR offers additional advantages in addition to the obvious enhancement of sensitivity, the unique potential for multiplex detection and an increased linear quantification range of the method is introduced. We show how various ELISA types suitable for... 

This experiment impressively demonstrated how an improved protein assay accessible by IPCR has significantly enhanced the opportunity for the early detection of potentially dangerous false-negative samples and emphasized the value of IPCR as a screening tool for low-level infections. Although IPCR sensitivity facilitates an early therapy, actual therapy monitoring and adaptation is supported by the broad dynamic quantification range. As the actual increase/decrease of the virus load is detectable in contrast to the simple qualitative presence/absence of the virus, detailed studies of therapy and medicament influences are possible. 

At the moment, validation (through the IRC, Association of Official Analytical Chemists, or multiple laboratory performance test) has not been performed on these kits. Our group has experience with the Soy Protein Residue Assay (Enhanced Assay) (ELISA Systems), which is used routinely for soy detection. This kit was one of the first available commercially for the detection and quantification of soy traces. We verified the applicability of the kit to a wide range of raw and processed matrices with good values for recoveries and reproducibility. Sensitivity is adequate to allergen detection in food (2.5 mg/kg as soy proteins). The extraction phase and subsequent steps of the immunoenzymatic assay are simple and fast. So far we have not found any problem... 

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