Self-assembled peptide-amphiphile nanofibers

Beniash E, Hartgerink JD, Storrie H et al (2005) Self-assembling peptide amphiphile nanofiber matrices for cell entrapment. Acta Biomater 1 387-397... 

Bull SR, Guler MO, Bras RE, Meade TJ, Stupp SI. Self-assembled peptide amphiphile nanofibers conjugated to MRI contrast agents. Nano Lett 2005 5 1-4. 

Hosseinkhani H, Hosseinkhani M, Tian F, Kobayashi H, Tabata Y. Osteogenic differentiation of mesenchymal stem cells in self-assembled peptide-amphiphile nanofibers. Biomaterials 2006 27 4079-86. 

Figure 6.2 Examples of static self-assembly, (a) Crystal structure of a ribosome, (b) Self-assembled peptide-amphiphile nanofibers, (c) An array of millimeter-sized polymeric plates assembled at a water/perfluorodecalin interface by capillary interactions, (d) Thin film of a nematic liquid crystal on an isotropic substrate, (e) Micrometer-sized metallic polyhedral folded from planar substrates, (f) A 3-D aggregate of micrometer plates assembled by capillary forces.

Hosseinkhani, H., M. Hosseinkhani, F. Tian, H. Kobayashi, and Y. Tabata. 2007. Bone r enera-tion on a coUagen sponge self-assembled peptide-amphiphile nanofiber hybrid scaffold. Tissue Eng 13 11-19. 

GaUer KM. et al. 2008. Self-assembling peptide amphiphile nanofibers as a scaffold for dental stem cells. Tissue Eng Part A 14(12) 2051-2058. 

Stupp, S.I., Hartgerink, J.D., and Niece, K.L. Self-Assembling Peptide-Amphiphiles and Self-Assembled Peptide Nanofiber Networks for Tissue Engineering, 2003-US29581 2004106359 (2004). 

Stupp, S.I., and Kessler, J.A. Self-Assembling Peptide Amphiphiles Generating Nanofiber Scaffolds for Encapsulation, Growth and Differentiation of Neurons for Therapeutic Uses, 2006-US2354 2006079036 (2006). 

Kim J, Anderson J, Jun H et al (2009) Self-assembling peptide amphiphile-based nanofiber gel for bioresponsive cisplatin delivery. Mol Pharm 6 978-985... 

Figure 73 Representative 3-D nanostructured scaffolds for bone-specific drug delivery systems, (a) Electrospun sitk scaffold with BMP-2 loaded, scale bar=5 pm (reprinted from Ref. [86] with permission) (b) Self-assembled peptide-amphiphile (PA) nanofibers network, scale bar= 1 mi (reprinted from Ref. [87] with permission) (c) Nanocrystalline apatite modified poly(lactide-co-glycolide) (PLAGA) microsphere scaffolds, scale bar=2pm (reprinted from Ref. [88] with permission) and (d) poly(L-lactic acid) (PLLA) nanofibrous scaffolds incorporated with poly(lactic-co-glycolic acid) (PLGA) nanospheres, scale bar=2 pm (reprinted from Ref. [89] with permission).

TambraUi, A., Blakeney, B., Anderson, J., et al. A hybrid biomimetic scaffold composed of electrospun polycaprolactone nanofibers and self-assembled peptide amphiphile ntmofibers. Biofabiication 1, 025001 (2009). doi 10.1088/1758-5082/l/2/025001... 

Figure 3.5 Schematics of the molecular structure (a) and of the resulting fibrous nanostructure (b) of a self-assembling peptide-amphiphile. In molecular graphics different atoms (C, H, O and N) are represented with different colors (black, white, red and blue), (c) TEM micrograph of nanofibers, and photograph of the macroscopic nanofiber sample. Adapted with permission from Ref. 72, Adv. Mater., 2005,17, 2612-2617. Doi 10.1002/ adma.200500855. Copyright 2005, Wiley-VCH Verlag GmbH Co. KGaA.

Hartgerink JD, Beniash E, Stupp SI (2001) Self-assembly and mineralization of peptide-amphiphile nanofibers. Science 294 1684—1688... 

Stendahl JC, Rao MS, Guler MO et al (2006) Intermolecular forces in the self- assembly of peptide amphiphile nanofibers. Adv Fund Mater 16 499-508... 

Paramonov SE, Jun H-W, Hartgerink JD. Self-assembly of peptide-amphiphile nanofibers the roles of hydrogen bonding and amphiphilic packing. J Am Chem Soc 2006 128 ... 

Figure 20.10. Amphiphilic ionic self-complementary peptides. This class of peptides has 16 amino acids, c. 5 nm in size, with an alternating polar and non-polar pattern. They form stable (3-strand and 3-sheet structures thus, the side chains partition into two sides, one polar and the other non-polar. They undergo self-assembly to form nanofibers with the non-polar residues inside positively and negatively charged residues form complementary ionic interactions, like a checkerboard. These nanofibers form interwoven matrices that further form a scaffold hydrogel with a very high water content ( 99.5%). The simplest peptide scaffold may form compartments to separate molecules into localized places where they can not only have high concentration, but also form a molecular gradient, one of the key prerequisites for prebiotic molecular evolution.

Hartgerin, D.K., Beniash, E., Stupp, S.I., 2002. Peptide-amphiphile nanofibers a versatile scaffold for the preparation of self-assembling materials. Proc. Natl. Acad. Sci. USA 99, 5133-5138. 

Fig. 7 Peptide Amphiphile Structure and Self-Assembly. From Intermolecular Forces in the Self-Assembly of Peptide Amphiphile Nanofibers by J.C. Stendahl, M.S. Rao, M.O Guler, and S.I. Stupp. Advanced Functional Materials, 6(4), p. 499. Copyright 2006 by Wiley-VCH Verlag GmbH Co. KGaA.

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