Sectioning technique paraffin tissue sections

A number of proteomic studies on archival material have utilized Liquid Tissue (Expression Pathology, Inc., Gaithersburg, MD), a commercial protein extraction kit for FFPE tissue.4,9,25-28 This kit is also based upon HIAR techniques and shares a similar work flow to the methods already discussed. Thin, typically 5-10pM, sections are cut from paraffin tissue blocks, the paraffin is removed, and the tissue deparaffinized and rehydrated in alcohols and distilled water before microdissection. The cellular material is then suspended in Liquid Tissue buffer and heated at 95°C for 90 min. Trypsin is added, and the material is digested overnight at 37°C prior to reduction with DTT and analysis by LC-MS/MS.26... 

A simple and effective AR technique of boiling archival paraffin-embedded tissue sections in water to enhance the signal of IHC was developed to circumvent the deleterious effects of formalin fixation, which had previously... 

Shi S-R, Liu C, Taylor CR. Standardization of immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval technique from experiments to hypothesis. J. Histochem. Cytochem. 2007 55 105-109. 

C. These freeze-dried sections were dry mounted on microscope slides which had been precoated with either Kodak NTB-3 or NTB-10 emulsion. Other techniques which thawed the frozen section, embedded the tissue in paraffin or dipped the section in liquid emulsion were demonstrated to translocate diffusible compounds. Many other similar attempts have been and are currently being made to localize diffusible compounds by autoradiography at the electron microscope level. 

Shi SR, Key ME, Kalra KL (1991) Antigen retrieval in formalin fixed, paraffin embedded tissues an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39 741 748 Shi SR, Cote RJ, Taylor CR (2001) Antigen retrieval techniques current perspectives. J Histo chem Cytochem 49 931 938... 

As previously mentioned, the principles of staining are identical to those used in paraffin sections. The techniques used may be direct or indirect as described in Chapters 15-18. Indirect techniques are generally more sensitive and, therefore, preferable. Indirect techniques can be broken down into three steps. In the first step, an antibody directed against the antigen of interest is applied to the tissue section. In the second step, a labeled secondary antibody directed against the first antibody is applied. The last step consists of a detection step that is composed of linking the secondary antibody to a detection system and... 

The method outlined here uses a modification of the Hedley technique (9,10) to prepare nuclear suspensions from the paraffin-embedded tissue samples. Microtome sections are dewaxed, hydrated, and incubated in pepsin with inter-... 

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