Secondary metabolites cultures

This is as far as we want to take our discussion of the development of deep cultures for the production of penicillin in this chapter. It is an aspect of the production of secondary metabolites, such as penicillin, more appropriately dealt with in the technology texts in the BIOTOL series. 

In the interdisciplinary field of biophysics and biotechnology, the bioeffects of electric field have received considerable interest for both fundamental studies on these interaction mechanisms and potential application. However, the effects of pulsed electric field (PEF) on secondary metabolism in plant cell cultures and fermentation processes have been unknown. Therefore, it would be very interesting to find out whether PEF could be used as a new tool for stimulating secondary metabolism in plant cell cultures for potential application to the value-added plant-specific secondary metabolite production. Furthermore, if the PEF permeabilization and elicitation are discovered in a cell culture system, the combination of... 

Howard, B. M., Nonomura, A. M. and Fenical, W. 1980. Chemotaxonomy in marine algae secondary metabolite synthesis by Laurencia in unialgal culture. Biochem. Syst. Ecol. 8 329-336. 

Schroeder, W. and Bohm, H., Once more secondary metabolite concentrations in whole plants and in cell cultures derived therefrom, J. Plant Physiol, 145,126,1995. Yang, R.Y.K. et al., Plant-cell bioreactors with simultaneous electropermeabilization and electrophoresis, J. BiotechnoL, 100, 13, 2003. 

J. Guern, J. P. Renaudin, S. C. Brown, The compartmentation of secondary metabolites in plant cell cultures. Cell Culture and Somatic Cell Genetics of Plants. Vol. 4 (F, Constabel and 1. K. Vasil, eds.). Academic Press, San Diego, 1987, p. 43. A. L. Samuels, M. Fernando, and A. D. M. Glass, Immunofluorescent localization of plasma membrane H -ATPase in barley roots and effects of K nutrition. Plant Physiol. 99 1509 (1992). 

W. Cisowski, W. Dembinska-Migas, M. Krauze-Baranowska, M. Luczkiewicz, P. Migas, G. Matysik and E. Soczewinski, Application of planar chromatography to the analysis of secondary metabolites in callus cultures of different plant species. J. Plan. Chromatogr.—Mod. TLC 11 (1998)441 146. 

Mulabagal V, Tsay H-S. (2004) Plant cell cultures — an alternative and efficient source for the production of biologically important secondary metabolites. Int J App Sci Eng 2 29 8. 

Mulabagal V, Lee C-Y, Lo S-F, Nalawade SM, Lin, C-Y, Tsay H-S. (2004) Studies on the production of some important secondary metabolites from medicinal plants by plant tissue cultures. Bot Bull Acad Sinica (Taiwan) 45 1-22. 

Plant cell culture is useful in laboratory and in industry because it allows plant natural products to be produced in a relatively controlled manner, and provides a supply of plant material that is not affected by sourcing problems, such as environmental, seasonal, geographical, and political factors.Also, plant cell culture allows for the tweaking and rearrangement of secondary metabolite biochemical pathways in order to produce novel metabolites, and to increase target compound yields, as well as allowing derivatives to be formed by introduction of analogs of natural intermediates.Plant cell culture can be performed with callus and suspension cultures, as well as with shoot cultures and hairy root cultures. These latter two approaches are especially useful when a metabolite is found to be produced more readily in differentiated cells. 

Rao SR, Ravishankar GA, Plant cell cultures Chemical factories of secondary metabolites, BiotechnolAdv 20 101—153, 2002. 

---