Plant cell cultures, synthesis secondary metabolites

Cell line selection is one of the traditional and effective approaches to enhancing metabolite accumulation, and biochemical studies provide the fundamental information for the intentional regulation of secondary metabolism in plant cells. In a carrot suspension culture regulated by 2,4-dichlorophenoxyace-tic acid, Ozeki et al. [7] found that there was a correlation between anthocyanin synthesis and morphological differentiation for somatic embryogenesis they also demonstrated the induction and repression of phenylalanine ammonia lyase (PAL) and chalcone synthase correlated with formation of the respective mRNAs. Two biosynthetic enzymes, i. e., PAL and 3-hydroxymethylglutaryl-CoA reductase, were also related with shikonin formation in Lithospermum erythro-rhizon cultures [8]. 

Somaclonal Variation and Secondary Product Synthesis. The induction and recovery of genetic variants by somaclonal variation technology can have a profound impact on the economic feasibility of secondary metabolite production. While most of the discussion up to this point has focused on cell culture production of secondary compounds, there are clearly a number of instances where whole plant production is both more efficient and economically prudent. This is especially true for those compounds... 

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