Seals glass based

To prepare CO solution for the experimental purpose, it is recommended to bubble 20 ml of stock solution in a sealed glass tube with a stream of pure CO gas. The bubbling process lasts for 20 min under the pressure of 100 kPa at 37°C [3]. One microliter of this CO-saturated solution is estimated to contain 30 ng of the gas based on the solubility of CO at 37°C, the extent of dilution of the CO-saturated solution, and the assumption that the loss of the added CO from the bath solution at the time of experiments is negligible. The stock solution of CO should be freshly prepared before each experiment and then should be diluted immediately to the desired concentration with the bath solution or culture media. 

Z.G. Yang, K.S. Weil, K.D. Meinhardt, J.W. Stevenson, D.M. Paxton, G.-G. Xia, and D.-S. Kim, Chemical compatibility of barium-calcium-aluminosilicate base sealing glasses with heat resistant alloys, in Joining of Advanced and Speciality Materials... 

Z. Yang et al., Chemical Compatibility of Barium-Calcium-Aluminosilicate-Based Sealing Glasses with the Ferritic Stainless Steel Interconnect in SOFCs, Journal of the Electrochemical Society, 150(8), pp. A1095-A1101 (2003). 

The earliest applications for quantitative analysis of liquid samples and solid preparations entailed sample dissolution in an appropriate solvent. A number of moisture determinations in APIs and pharmaceutical preparations based on both reflectance and transmission measurements have been reported. Their results are comparable to those of the KF method. The high sensitivity provided by the NIR technique has fostered its use in the determination of moisture in freeze-dried pharmaceuticals. ° The noninvasive nature of NIR has been exploited in determination of moisture in sealed glass vials. " " ... 

A newer technique which shows promise but has not yet been fully tested is based upon the preparation and separation of aldonitrile acetates of reducing sugars (Equation 9.12). The dried sugar is dissolved in pyridine and treated with hydroxylamine hydrochloride in a sealed glass ampoule at 90°C for 0.5 hr. The mixture is cooled, acetic anhydride added, and another 30-min reaction is carried out in the resealed ampoule. Actual formation of the nitrile may occur in the injector of the gas chromatograph. Nonreducing sugars apparently do not form the nitrile but are present as the... 

A solution or dispersion consisting of 20.1 g (0.1 mol) of y-chloro-p-fluorobutyrophenone, 19.8 g (0.2 mol) of 4-methylpiperidine and 0.1 g of potassium iodide in 150 ml toluene is heated in a sealed glass tube for 15 hours at 100°C to 110°C. The potassium iodide and the 4-methylpiperidine hydrochloride formed in the reaction are separated by filtration and the solvent removed from the filtrate by evaporation in vacuum on a steam bath. The residue is distilled and the fraction obtained at 120°C to 125°C and at a pressure lower than 0.1 mm Hg is collected. The base is dissolved in ether and the 4-fluoro-y-(4-methylpiperidino)-butyrophenone precipitated as the hydrochloride. The reaction product is purified by recrystallization in ethanol/ether. 

The planar chips used by the PatchXpress, QPatch, and Patchliner systems incorporate a glass-based substrate that permits the formation of GQ-level seal resistances between the cells and the plate. Consequently, the noise level on these systems tends to be low and they are thus capable of distinguishing small ionic currents (<100 pA) reliably. Their planar chips contain 8, 16, or 48 wells with a single aperture in the center bottom position of each well. These systems permit parallel recordings from several individual cells on the chip. However, due to variation in the performance of randomly selected cells, only a proportion are expected to complete any given experimental protocol and so the capacity to acquire pharmacology data may not match initial expectations (Xu et al. 2003). 

Synthesis of 7,12-Dioxaspiro (5,6)-dodec-8-ene (III). Monomer III (50.4g, 0.3 mol), was combined with 30 ml tert-butyl alcohol and 15g (0.14 mol) of potassium tert-butoxide. The mixture was heated in a sealed glass reactor for 6 hr. at 120°C. After cooling, the solution was diluted with diethyl ether and extracted with water to remove base. Evaporation of the diethyl ether and distillation gave a crude mixture of II and III as a water white liquid, bp 67-69.5°C/lmm [lit. 76-76.5°C/8mm for III (21)]. The NMR spectrum with peaks at 66.07, 65.66, 64.83, 64.24, 63.85, 62.28 and 61.9-1.3ppm, indicated that the II/III mixture contained ca. 

Randolph s tests with alkaline phosphatase were carried out in a stirred autoclave. An amount of the disodium salt and some water, which is required for the enzyme catalyzed hydrolysis, were placed in the autoclave along with a sealed glass ampule containing the enzyme. In this case water is necessary not just to render the enzyme active, as Klibanov found, but also to serve as a reactant in the hydrolysis. Carbon dioxide was admitted, the temperature and pressure adjusted to the level desired, and the sealed ampule shattered to expose the enzyme and to mark the zero point of the reaction sequence. In their studies they investigated the effects of changing the relative amount of enzyme on the rate of conversion of the disodium salt of p-nitrophenyl phosphoric acid to p-nitrophenol. They measured the amount of conversion by UV analysis of the solution removed from the autoclave at the end of a reaction test. The results are shown in Figure 11.1 based upon these results and other experimental results, the authors concluded that the rate-determining step of the enzyme-catalyzed reaction was the dissolution of disodium p-nitrophenyl phosphate in supercritical carbon dioxide. 

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