Screening methods mass spectrometric

Selecting an approach A nearby lab specializes in mass spectrometric analysis and can perform the EPA screening method for pesticides and other toxic chemicals. Your own lab just bought an inductively coupled plasma emission spectrometer and can analyze the water for heavy metals. 

Liao W, Joe T, Cusick WG. 1991. Multiresidue screening method for fresh fruit and vegetables with gas chromatographic/mass spectrometric detection. J Assoc Off Anal Chem 74(3) 554-565. 

The use of 96-well microtiter plates coupled with the rapid quantification by a spectrophotometric plate reader allows this system to screen a large array of compounds in a relatively short period of time. Alternatively, the compound analysis can be performed with fast and sensitive LC/MS by pooling the samples together. Both the spectrophotometric and mass spectrometric methods... 

Solubility screens using LC/MS detection do not require an ultra-pure sample of the test compound due to the selective detection of the mass spectrometer. Mass spectrometric detection offers high selectivity and low detection limits, which eliminates the need to develop complex chromatographic methods. The LC/MS-based solubility screen surpasses the traditional HPLC/UV-based equilibrium solubility assay with increased throughput, minimal manual intervention, and high sensitivity and selectivity. 

A number of indirect methods have been developed with mass spectrometric detection to rapidly study non-covalent complexes for drug screening purposes [2]. Among the most promising and simple indirect methods that overcome the limitations described above for directly studying non-covalent complexes by mass spectrometry is the application of size exclusion techniques in the spin column format for the screening and analysis of drug-protein complexes under optimum mass spectral sensitivity conditions [11-13]. 

L., Hindsgaul, O. Micro-scale frontal affinity chromatography with mass spectrometric detection A new method for the screening of compound libraries. Angew Chem Int Ed 1998, 37, 3383-3387. 

Mass spectrometric methods are fast and can be automated. Thus, they are ideal methods for high throughput screening. Compared to ESI-MS, MALDI-MS shows typically higher tolerance against impurities like buffers, for example. Therefore, samples can be measured which are directly taken from a test solution without further pretreatment. On the other hand, electrospray mass... 

Wolfender, J.-L. and Hostettmann, K., Liquid chromatographic-UV detection and liquid chromatographic-thermospray mass spectrometric analysis of Chironia (Gentianaceae) species. A rapid method for the screening of polyphenols in crude plant extracts, J. Chromatogr. A, 647, 191, 1993. 

Gladys et al. (620) described a gas chromatographic-mass spectrometric method for screening and confirmation of residues of the thyreostatic substances... 

Conventionally, metabolite identification and more specifically reactive metabolite screening typically uses an array of chromatographic and mass spectrometric methods, and may require multiple injections of the same sample. This is to ensure that enough information has been collected to detect all metabolites and to have sufficient fragmentation information available to elucidate structures. 

Gael, D., P. Gille, D. Fraisse, and D. Barcelo. (1992). Comparison of gas chromatrographic-mass spectrometric methods for screening of chlorotriazine pesticides in soil. J. Chromatogr. A, 602 175-184. 

Goolsby, D.A., E.M. Thurman, M.L. Clark, and M.L. Pomes (1991). Immunoassay as a screening tool for triazine herbicides in streams, comparison with gas chromatographic-mass spectrometric methods. In M. Vanderlaan., L.H. Stanker, B. Watkins, and D.W. Roberts, eds., Immunoassays for Trace Chemical Analysis. ACS Symposium Series 451. Washington, DC American Chemical Society, pp. 86-99. 

Harada, K., Murata, H., Qiang, Z., Suzuki, M., and Kondo, F. 1996a Mass spectrometric screening method for microcystins in cyanobacteria. Toxicon 34 701—710. 

Labeling proteins with heavy and light tags and screening the hit compound versus an inactive control, followed by mass spectrometric comparison of the two samples, is another approach that avoids many of the common pitfalls in affinity methods (3). Techniques such as stable isotope labeling with amino acids in cell culture and isotope-coded affinity tagging (ICAT) exemplify these techniques. 

The advantage of this screening method over other approaches is the elimination of purification steps before mass spectrometric identification. Also, the disadvantages associated with chromatographic separations are eliminated. However, the use FTICR mass spectrometric screening restricts the binding... 

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