Scotch-Brite

Abrade Hand abrade with Scotch-Brite wet with MEK until the surface is shiny. Use fine-grit, dry Scotch-Brite - pads on high-speed grinder driven by dry. oil-free N2 until the surface is shiny. 

All AS-4/APC-2 surfaces were cleaned with SCOTCH-BRITE and BON AMI before the surface treatment. 

Place the gel holder and Scotch Brite pads in the biggest vessel, and six sheets of Whatman paper in another vessel The size of the Whatman sheets should be slightly smaller than the size of the Scotch Brite pads... 

Put a wetted Scotch Brite pad on one side of the gel holder, and then three sheets of Whatman paper saturated with transfer buffer on top of the pad. 

Next place three Whatman sheets prewetted with Tris-glycine buffer on top of the PVDF membrane and finally a prewetted second Scotch Brite pad. Close the holder. 

Soak the nitrocellulose membrane for 15-20 min in transfer buffer. Also wet two Scotch-Brite fiber pads, gel and filter papers in transfer buffer. 

Scotch-Brite pads (aze depends on the size of the gel holder). 

Place the gel holder in one of the dishes. Put a wetted Scotch-Brite pad on one side of the holder and then three sheets of Whatman paper saturated with transfer buffer on the pad. Place the gel on the paper direcdy after taking it out from the electrophoresis apparatus. The upper stacking gel should be carefully removed because it sticks to nitrocellulose. Pour a few milliliters of transfer buffer on top of the gel and carefully place on it a sheet of prewetted nitrocellulose. Roll over the nitrocellulose with a glass rod to remove any air bubbles between the gel and the membrane. Next, place three Whatman 3MM sheets prewetted with transfer buffer on top of the nitrocellulose, and finally another wet Scotch-Brite pad. 

If your paper feed jams are caused by worn pickup rollers, there is something you can do to get your printer working while you re waiting for the replacement pickup rollers. Scuff the feed roller(s) with a Scotch-Brite pot-scrubber pad (or something similar) to roughen up the feed rollers. This trick only works once. After that, the rollers aren t thick enough to touch the paper. 

The first step in fabricating the PC board electrodes is to clean each board thoroughly. You can use a Scotch Brite pad and water to clean the circuit board. Even though the circuit board looks shiny and very clean, rub it all over with the pad and then rinse with water. 

When the etching is complete, remove the blue colored resist by scrubbing it off with a Scotch Brite type pad under running water. As you scrub, the copper trace will reveal itself. Remove all the resist and the plates will be done. 

Pry the glass plate from the gel (which remains on other plate) and cover the gel carefully with filter paper (no air bubbles). This facilitates the handling of the gel (sticks to the paper). Lift the gel carefully by the paper from the glass plate and place it (gel side up) on a Scotch-Brite pad with a 3MM filter paper. 

Wet the gel with a small amount of TBE and place the nylon membrane carefully on the gel. Place successively two 3MM filter papers (soaked for at least 2 h in TBE) on top of the membrane and cover with a second Scotch-Brite pad. Air bubbles should be avoided during each addition of membrane or filter paper (otherwise remove by rolling pipette). 

Place filter paper gel-side up on top of maroon Scotch-Brite pads sitting on first white grill of transfer apparatus and wet liberally with. 5x TBE. 

Place other maroon Scotch-Brite pads on top along with second white grill and turn upside down so that labeled transfer membrane faces down and filter paper is on top. Rubber band each side. 

There are many choices for wiper media. It can be felt, cloth, indoor carpet, outdoor carpet, leather, Scotch-Brite, or any similar material in sheet form. Care should be taken when selecting the basic unit to allow for quick and easy changing of the wiper media, as a conscientious operator will change the wipers frequently to ensure consistency in the winding tension. Another technique used... 

Composite abrasive materials, such as Scotch-Brite (3M Company), have proved popular for mechanical surface preparation. These commercial abrasive materials are available in pad and sheet form, and they can conform easily to the shape of a surface. When combined with water flushing, they provide clean, almost oxide-free surfaces. Hand sanding, wire brushing, and other abrasion methods that are highly related to the operator s skill, and patience must be carefully controlled. These processes are a source of inconsistency and should be used only when no other method is possible. 

Assemble the transfer sandwich Scotch Brite pad (or equivalent scouring pad), three layers of Whatman 3MM paper, the gel, the membrane (nitrocellulose, nylon, or Immobilon-P—the latter has to be prewetted in methanol), three layers of 3MM paper, and another Scotch Brite pad. Transfer gel-fractionated proteins electrophoretically to a membrane under conditions that have been experimentally determined to be best for the protein of interest. With a Bio-Rad Trans-Blot apparatus, transfer of a 60-kDa protein can be accompUshed using 60 V for 1 hr. 

Substrate was 2024 T3 bare aluminum, solvent-wiped, Scotch-Brite -abraded, and solvent-wiped bonds were aged three days at RT, then half were aged an additional seven days at 90°C. These OLS bonds were tested at 23°C according to ASTM D1002-72. 

Carbon fiber-polyether ether-ketone (PEEK ) Solvent wipe with MEK. Abrade lightly with Scotch-brite-Bon Ami. Good strengths require (A) or (B) treatment Rinse in tap water and DI water. Airdiy. 

Specimens As-received aluminum lapshear prepared with 3M Scotch-Brite pads with reagent-grade acetone wash. 

Abrasive surfaces - e.g. sandpaper, emery paper, steel wool, Scotch-brite and Soft-scour scouring pads, etc. 

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