Sanger sequencing

Finally, if no reference genome is available, one can try to assemble the raw read sequences to regions of enrichment. A combination of Sanger sequencing and next-generation sequencing may be helpful here. 

As a result, 16S sequences with long homopolymers are still better handled by, with the exception of Ion Torrent, other technologies, such as Sanger sequencing. 

This technique uses a combination of SSCP and Sanger sequencing to create a larger set of bands for analysis in a native acrylamide gel (7). In this method... 

Sanger sequencing. A widely used method of determining the order of bases in DNA. 

In principle, the concept behind NGS technology is similar to classic Sanger sequencing the bases of a fragment of DNA are sequentially identified from signals emitted as each fragment is resynthesized from a DNA template... 

The first sequencing methods used Sanger sequencing of cDNA or EST (expressed sequence tag) libraries [28], but they were low throughput and not quantitative. Currently various... 

Sanger sequencing is the most common technique used in molecular laboratories to detect the exact nucleotide composition of PCR-amplified DNA fragments. It uses chain-terminating dideoxynucleoside triphosphates... 

Sanger, Sequences, Sequences, and Sequences, Annual Reviews of Biochemistry 57 1-28,1988. JP. Swann, Academic Scientists and the Pharmaceutical Industry Cooperative Research in Twentieth Century America (Baltimore, MD The... 

The Sanger sequencing method is outlined here. The principle depends on the ability of an enzyme, DNA polymerase, to make a very accurate complementary... 

Primer and template in Sanger sequencing. The cartoon, panel 1, shows a long single strand of DNA (labeled "template") annealed to a primer that is complementary in sequence to part of the template. DNA polymerase has already synthesized a "new strand" of DNA, starting from the 30 end of the primer and continuing along the template strand until a dideoxynucleotide was incorporated. The polymerase is now stopped as there is no 30-OH... 

Sanger sequencing gel result. An autoradiograph of a gel from a Sanger sequencing experiment. The nucleotide sequence can be read directly from the gel, as shown on the right of the gel. 

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