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Sanger dideoxy sequencing technique

Rapid sequencing techniques have been developed to further the analysis of DNA molecules. DNA can be sequenced by controlled interruption of replication (Sanger dideoxy method). The fragments produced are separated by gel... [Pg.270]

Small DNA segments can be synthesized in the laboratory, and commercial instruments are available for automating the work. Sequencing of DNA can be carried out either by the Maxam-Gilbert method, which uses chemical techniques, or by the Sanger dideoxy method, which uses enzymatic techniques. Small amounts of DNA can be amplified by factors of 10 using the polymerase chain reaction (PCR). [Pg.1187]

Southern and Northern blotting techniques (p. 137) Sanger dideoxy method of DNA sequencing (p. 138) Solid-phase synthesis of nudeic acids (p. 139)... [Pg.1129]

Which of the following sets of reagents are required for dideoxy chain DNA synthesis in the Sanger technique for DNA sequencing . ... [Pg.316]


See other pages where Sanger dideoxy sequencing technique is mentioned: [Pg.1112]    [Pg.466]    [Pg.379]    [Pg.1112]    [Pg.1174]    [Pg.1194]    [Pg.1112]    [Pg.138]    [Pg.1174]    [Pg.476]    [Pg.999]    [Pg.1140]    [Pg.357]    [Pg.293]    [Pg.146]    [Pg.50]    [Pg.238]   
See also in sourсe #XX -- [ Pg.304 ]




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