Sequencing methods Sanger protocol

Two basic protocols for DNA sequencing are commonly adapted in laboratories the chemical cleavage method (Maxam and Gilbert, 1977) and the enzymatic chain termination or dideoxy method (Sanger et al, 1977). Of the two approaches, the Sanger dideoxy... 

The sequencing of DNA can be accomplished by using both chemical (introduced by Gilbert with his student Maxam) and enzymatic methods, especially the Sanger dideoxy-nucleotide protocol. In both approaches, and in analogy to protein analysis (Section 26-5), the unwieldy DNA chain is first cleaved at specific points by enzymes called restriction endonucleases. 

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