Sanger DNA sequencing

F Sanger. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74 5463-5467, 1977. 

Fig. 6.22. The Sanger DNA-sequencing procedure, (a) The Sanger dideoxynucleotide sequencing reaction with 32p. abeled primers, (b) Acrylamide gel separation of the labeled fragments. (Adapted from Watson JD, Gilman M, Witkowski J, et al. Recombinant DNA, 2nd Ed. New York Scientific American Books, W.H. Freeman and Company, 1992 with permission.)...

Blazej, R. G., Kumaresan, R, and Mathies, R. A. Microfabricated bioprocessor for integrated nanoUter-scale Sanger DNA sequencing. Proceedings of the National Academy of Sciences of the United States of America 103, 7240-7245 (2006). 

Blazej RG, Kumaresan P, Mathies RA (2006) Microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencing. Proc Natl Acad Sci U S A 103(19) 7240-7245... 

Blazej et al. [7] also reported a microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencing (Fig. 4). The bioprocessor is able to conduct all three Sanger sequencing steps, thermal cycling, sample purification, and capillary electrophoresis. They were able to... 

Sanger dideoxy method (Section 2.6) The most commonly used method of DNA sequencing. 

DNA Sequencing The following DNA fragment was sequenced by the Sanger method. The red asterisk indicates a fluorescent label. 

---