Saccharomyces cerevisiae simultaneous

Keywords Saccharomyces cerevisiae Simultaneous saccharification and fermentation ... 

A trivial yet important application is following ethanol production via a bioprocess. Sivakesava et al.1 simultaneously measured glucose, ethanol, and the optical cell density of Saccharomyces cerevisiae during ethanol fermentation, using an off-line approach. Samples were brought to an instrument located near the fermentation tanks and the measurements made in short order. While they eventually used MIR due to the interfering scatter of the media, they proved that Raman could be used for this application. 

The fifth paper, "A Separative Bioreactor Direct Product Capture and pH Control," presented by Seth Snyder of the Argonne National Laboratory, reviewed development and performance of a novel bioreactor incorporating electrodeionization to simultaneously produce and separate products from both enzymatic and microbially mediated reactions. The sixth paper, " Optimization of Xylose Fermentation in Spent Sulfite Liquor by Saccharomyces cerevisiae 259ST," presented by Steven Helle of the University of British Columbia, provided an overview of an approach to fermentation optimization utilized to identify key process variables limiting use of the SSL for commercial ethanol production. 

Simultaneous saccharification and fermentation (SSF) experiments were conducted according to NREL standard protocol (LAP-008). Each SSF flask was loaded with 3% (w/w) glucan, 1% (w/v) yeast extract, 2% (w/v) peptone, 0.05 M citrate buffer (pH 4.8), the appropriate amount of cellulase enzyme to achieve 15 FPU/g of glucan, and the appropriate amount of Saccharomyces cerevisiae DSA (provided by NREL) inoculum (starting optical density of 0.5). The SSF flasks were equipped with water traps to maintain anaerobic conditions and were incubated at 37°C with gentle rotation (130 rpm) for a period of 168 h. 

Karimi, K., Emitiazi, G., and Taherzadeh, M. J. 2006. Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae. Enz Microbial Technol., 40,138-144. 

Ohta, K., Hamada, S., and Nakamura, T. 1993. Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae. App. Environ. Microbiol, 59, 729-733. 

When Saccharomyces cerevisiae grow in a high sugar concentration, as is found in grape juice, their mitochondria degenerate. Simultaneously, the enzymes of the... 

Mashego, M., Van Gulik, W., and Heijnen, J. 2006. Metabolome dynamic responses of Saccharomyces cerevisiae to simultaneous rapid perturbations in external electron acceptor and electron donor. FEMS Yeast Res. 7(1), 48-66. 

Chandrakant, R, Bisaria, V. S. (2000). Simultaneous bioconversion of glucose and xylose to ethanol by Saccharomyces cerevisiae in the presence of xylose isomerase. Applied Microbiology and Biotechnology, 53, 301-309. 

Lebeau T., Jouenne T. and Junter G.-A. Simultaneous fermentation of glucose and xylose by pure and mixed cultures of Saccharomyces cerevisiae and Candida shehatae immobilized in a two-chambered bioreactor. Enzyme and Microbial Technology 21 (1997) 265-272. 

Servetas I., Berbegal C., Camacho N., Bekatorou A., Ferrer S., Nigam P, Drouza C. and Koutinas A.A. Saccharomyces cerevisiae and Oenococcus oeni immobilized in different layers of a cellulose/starch gel composite for simultaneous alcoholic and malolactic wine fermentations. Process Biochemistry 48 (9) (2013) 1279-1284. 

Gobbi, M., Comitini, R, Domizio, R, Romani, C., Lencioni, L., Mannazzu, I., et al. (2013). Lachancea thermotolerans and Saccharomyces cerevisiae in simultaneous and sequential co-fermentation a strategy to enhance acidity and improve the overall quality of wine. Food Microbiology, 33, 271-281. http //dx.doi.Org/10.1016/j.fm.2012.10.004. 

Saccharomyces cerevisiae is the dominant microorganism in the first generation of fuel ethanol production. In recent years, the worldwide bioethanol production reached around 80 billion liters per year. In a typical industrial scale bioethanol fermentation process using Saccharomyces cerevisiae, around 8-14% (v/v) ethanol is produced and the glucose to bioethanol yield is usually over 90% of the theoretical yield. In some processes, simultaneous saccharification and fermentation is applied, in which a-amylase/glucoa-mylase is mixed with Saccharomyces cerevisiae and starchy raw materials. Most of yeast cells harvested in the fermentation are recycled and sent back in order to enhance the cell concentration in the fermenter. Around 5-10% yeast cells end up in Dried Distillers Grains with Solubles (DDGS), which could be sold as animal feed. 

Ha SJ, Galazka JM, Kim SR, Choi JH.Yang X, Seo JH, et al. Engineered Saccharomyces cerevisiae capable of simultaneous ceUobiose and xylose fermentation. Proc Natl Acad Sci USA 2013 108 504-9. 

Yokotsuka, K., a. Otaki, A. Naitoh, and H. Tanaka. 1993. Controlled simultaneous deacidification and alcohol fermentation of a high-acid grape must using two immobilized yeasts, Schizosaccharomyces pombe and Saccharomyces cerevisiae. Am.]. Enol. Vitic. 44 371-377. 

The enzymatic activity and ethanol formation abilities of Fusarium oxysporum makes it ideal for a simultaneous saccharification and biological conversion process [82]. Birch wood containing high xylose content, was used for the simultaneous saccharification and biological conversion test [82]. The birch chips were steam-treated at 185 °C for 30 min. Cellulase from Trichoderma reesei and either Saccharomyces cerevisiae or Fusarium oxysporum were used for the simultaneous saccharification and biological conversion process. Rates of hydrolysis and bioconversion were studied by varying the amount of T. reesei cellulase, since the... 

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