Saccharomyces cerevisiae process

Aksu Z (2003) Reactive dye bioaccumulation by Saccharomyces cerevisiae. Process Biochem 38 1437-1444... 

Saccharomyces cerevisiae processing High yields (g/l in Pichia pastoris) Inexpensive Formation of disulphides Glycosylation mammalian systems... 

Lipari, F., B. J. Gour-Salin, and A. Herscovics, The Saccharomyces cerevisiae processing a 1,2-mannosidase is an inverting glycosidase, Biochem. Biophys. Res. Commun., 1995, 209, 322-326. 

Burke, J., F. Lipari, S. Igdoura, and A. Herscovics, The Saccharomyces cerevisiae processing al,2-mannosidase is localized in the endoplasmic reticulum, independently of known retrieval motifs. Ear. J. Cell Biol, 1996, 70, 298 305. 

Millati, R., Niklasson, C., Taherzadeh, M., 2002. Effect of pH, time and temperature of over-hming on detoxification of dilute-acid hydrolyzates for fermentation by Saccharomyces cerevisiae. Process Biochemistry 38 (4), 515—522. 

In current industrial practice, benzaldehyde is added to fermenting baker s yeast Saccharomyces cerevisiae) with resultant PAC production occurring from the yeast-derived pyruvate. Typically PAC concentrations of 12-15 g F are produced at yields of 65-70% theoretical in a 10-12 h biotransformation process. [2], Appreciable concentrations of benzyl alcohol are produced as by-product due to oxidoreductase activity in the fermentative yeast. 

Wright R.M., Repine T. Repine J.E. (1993) Reversible pseudohyphal growth in haploid Saccharomyces cerevisiae is an aerobic process. CurrGenet, 23, 388-391. 

The accumulation of a number of amino acids from the external medium seems almost irreversible in Saccharomyces cerevisiae. The first detailed study of this phenomenon concerned histidine [13]. Histidine uptake by the specific histidine permease HlPl is an energy dependent process which accumulates free and intact... 

Feedback inhibition of amino acid transporters by amino acids synthesized by the cells might be responsible for the well known fact that blocking protein synthesis by cycloheximide in Saccharomyces cerevisiae inhibits the uptake of most amino acids [56]. Indeed, under these conditions, endogenous amino acids continue to accumulate. This situation, which precludes studying amino acid transport in yeast in the presence of inhibitors of protein synthesis, is very different from that observed in bacteria, where amino acid uptake is commonly measured in the presence of chloramphenicol in order to isolate the uptake process from further metabolism of accumulated substances. In yeast, when nitrogen starvation rather than cycloheximide is used to block protein synthesis, this leads to very high uptake activity. This fact supports the feedback inhibition interpretation of the observed cycloheximide effect. 

The regulation of NCR-sensitive amino acid transporters in Saccharomyces cerevisiae has many points in common with that of catabolic enzymes. Amino acid permeases, as well as some other transporters of nitrogenous nutrients, are integrated into the regulatory circuits, both general and specific, which control catabolic processes. 

Remsen-Fahlberg process for, 24 235 sodium nitrite in, 22 860 Saccharomyces, 26 445, 446 Saccharomyces carlsbergensis, 3 580 Saccharomyces cerevisiae, 12 478—479, 26 446, 448, 449, 3 580. See also Bakers yeast... 

In another fermentation process, Mosheky et al.18 reacted Saccharomyces cerevisiae with sugars and followed the progress of the fermentation with MIR-ATR. Two PLS models were used one for sucrose, fructose, and glucose and one for the ethanol. The authors did not specify SEPs for the experiment, but showed correlation coefficients of better than 0.998 for all analytes. 

The four antigens are produced in a fermentation process using the yeast Saccharomyces cerevisiae grown in chemically defined media. The purified antigens are formulated in aluminum-containing adjuvant in sterile liquid suspension. 

Yeast and fruit are input variables in the wine-making process. In the case of yeast, the amount of a given strain could be varied, or the particular type of yeast could be varied. If the variation is of extent or quantity (e.g., the use of one ounce of yeast, or two ounces of yeast, or more) the variable is said to be a quantitative variable. If the variation is of type or quality (e.g., the use of Saccharomyces cerevisiae, or Saccharomyces ellipsoideus, or some other species) the variable is said to be a qualitative variable. Thus, yeast could be a qualitative variable (if the amount added is always the same, but the type of yeast is varied) or it could be a quantitative variable (if the type of yeast added is always the same, but the amount is varied). Similarly, fruit added in the wine-making process could be a qualitative variable or a quantitative variable. In the algebraic system, x is a quantitative variable. 

In reviews on the use of in situ sensors" or optical sensor systems" for bioprocesses, UV-vis does not play a major role. An example for the application of at-line UV-vis spectroscopy was presented by Noui et al. The selective flocculation processes of Saccharomyces cerevisiae homogenates were followed with a newly developed direct UV spectrophotometer. The results from a PLS regression model were in good agreement with those from off-line chemical assays. 

Holz, C., Hesse, O., Bolotina, N., Stahl, U. and Lang, C. (2002). A micro-scale process for high-throughput expression of cDNAs in the yeast Saccharomyces cerevisiae. Protein Expr. Purif. 25, 372-378. 

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