Saccharomyces cerevisiae kinetics

In this chapter, we shall focus on the molecular aspects of amino acid transport and its regulation in Saccharomyces cerevisiae. Kinetic, biochemical and genetic aspects of the amino acid transport systems of eukaryotic microorganisms have been reviewed earlier [7,8]. 

Buxeda, R.J. Nickels, J.T. Belimis, C.J. Carman, G.M. Phosphatidylinositol 4-kinase from Saccharomyces cerevisiae. Kinetic analysis using Triton X-100/phosphatidylinositol-mixed micelles. J. Biol. Chem., 266, 13859-13865... 

Nika, J., Yang, W., Pavitt, G. D., Hinnebusch, A. G., and Hannig, E. M. (2000). Purification and kinetic analysis of eIF2B from Saccharomyces cerevisiae. J. Biol. Chem. 275, 26011-26017. 

Gadd, G. M. and Laurence, O. S. (1996). Demonstration of high-affinity Mn2+ uptake in Saccharomyces cerevisiae specificity and kinetics, Microbiol., 142, 1159-1167. 

D. Visser, R. van der Heijden, K. Mauch, M. Reuss, and S. Heijnen, Tendency modeling A new approach to obtain simplified kinetic models of metabolism applied to Saccharomyces cerevisiae. Metab. Eng. 2(3), 252 275 (2000). 

J. L. Galazzo and J. E. Bailey, Fermentation pathway kinetics and metabolic flux control in suspended and immobilized Saccharomyces cerevisiae. Enzyme Microb. Technol. 12(3), 162 172 (1990). 

Jong, A.Y.S. Campbell, J.L. Characterization of Saccharomyces cerevisiae thymidylate kinase, the CDC8 gene product. General properties, kinetic analysis, and subcellular localization. J. Biol. Chem., 259, 14394-14398 (1984)... 

Zook, C.D., Parish, M.E., Braddock, R.J. and Balaban, M.O. (1999) High pressure inactivation kinetics of Saccharomyces cerevisiae ascospores in orange and apple juices. Journal of Food Science 64(3), 533-5. 

You are going to cultivate yeast, Saccharomyces cerevisiae, by using a 10 m -fermenter your company already owns. You want to find out the amount of ethanol the fermenter can produce. Therefore, a chemostat study was carried out and the Monod kinetic parameters for the microorganism grown in the glucose medium at 30°C, pH 4.8, were found to be Ks = 0.0025 g/L and /imax = 0.25 h-1. The ethanol yield (YP/S) is 0.44 (g/g) and cell yield (Yx/S) is 0.019 (g/g). The inlet substrate concentration is 50 g/L-... 

Despite the large number of available PDC-genes, only a few enzymes have been characterized in detail with regard to their protein stability, relevance of functional groups and kinetic properties. The enzymes from Saccharomyces cerevisiae [34,40-47,62-64,74,75,77-80,83,107-116] and Zymomonas mobilis [72,76,82,106,117-119], and Pisum sativum [125,126] have been characterized in detail. Some data are available for the enzymes from wheat germ... 

A set of alcoholic fermentations experiments was conducted in order to verify the performance of the model-based substrate sensor. Diluted molasses was used in the experiments as feed substrate and bread yeast, Saccharomyces cerevisiae, as inoculum. The operational conditions and kinetic parameters used are given in Table 1 for two different experiments (tests 1 and 2), and values of ethanol and biomass concentrations were also determined off-line using gas chromatography (CG) and dry cell weight standard (7) methods, respectively. 

Kinetics of Asparaginase II Fermentation in Saccharomyces cerevisiae ure2dal80 Mutant... 

Index Entries Saccharomyces cerevisiae ure2dal80 mutants nitrogen sources asparaginase II fermentation kinetics. 

The present study investigated the inhibition of Saccharomyces cerevisiae by the liquid hydrolysate and the kinetics of enzymatic hydrolysis of the solid components produced by the pretreatment of aspen wood and corn stover by liquid hot water and hot carbonic acid. Inhibition of yeast was determined by measuring the rate of glucose consumption by yeast growing in hydrolysates produced at various reaction severities. The enzymatic hydrolysis rates of pretreated solids was determined by measuring rates of sugar accumulation of enzyme-digested pretreated solids. 

Vanadate-stimulated NAD(P)H oxidation activity was first reported in the erythrocyte membrane [20] and has been found in widely diverse membranes including mammalian rat liver [21], the sugar beet plant [22], and the fungus Saccharomyces cerevisiae [23] membrane. The kinetics of NADH oxidation [24] observed in the presence of vanadate and plasma membranes show a variable lag, with H202 and... 

Biosimulation has a dominant role to play in systems biology. In this chapter, we briefly outline two approaches to systems biology and the role that mathematical models has to play in them. Our focus is on kinetic models, and silicon cell models in particular. Silicon cell models are kinetic models that are firmly based on experiment. They allow for a test of our knowledge and identify gaps and the discovery of unanticipated behavior of molecular mechanisms. These models are very complicated to analyze because of the high level of molecular-mechanistic detail included in them. To facilitate their analysis and understanding of their behavior, model reduction is an important tool for the analysis of silicon cell models. We present balanced truncation as one method to perform model reduction and apply it to a silicon cell model of glycolysis in Saccharomyces cerevisiae. 

T. V. Kulakovskaya, N. A. Andreeva, A. V. Karpov, I. A. Sidorov and I. S. Kulaev (1999). Hydrolysis of tripolyphosphate by purified exopolyphosphatase of Saccharomyces cerevisiae cytosol kinetic model. Biochemistry (Moscow), 64, 990-993. 

Hassett, R. E, Yuan, D. S., and Kosman, D. J. (1998). Spectral and kinetic properties of the Fet3 protein from Saccharomyces cerevisiae, a multinuclear copper ferroxidase enzyme. J. Biol. Chem. 273, 23274-23282. 

Tegoni, M., Silvestrini, M. C., Guigliarelli, B., ASSO, M., Brunori, M., and Bertrand, P., 1998, Temperature-jump and potentiometric studies on recombinant wild type and Y143F and Y254F mutants of Saccharomyces cerevisiae flavocytochrome b2 role of the driving force in intramolecular electron transfer kinetics. Biochemistry 37 12761nl2771. 

Jemec, K. R, Raspor, P. (2005) Initial Saccharomyces cerevisiae concentration in single or composite cultures dictates bioprocess kinetics. Pood Microbiology, 22, 293-300. 

Rosenfeld, E., Beauvoit, B., Blondin, B., Salmon, J. M. (2003) Oxygen consumption by anaerobic Saccharomyces cerevisiae under enological conditions Effect on fermentation kinetics. Applied and Environmental Microbiology, 69, 113-121. 

A.J. Jackson-Fisher, et al. Dimer dissociation and thermosensitivity kinetics of the Saccharomyces cerevisiae and human TATA binding proteins. Biochemistry 38 (1999) 11340-8. 

V. Petri, M. Hsieh, E. Jamison and M. Brenowitz. DNA sequence-specific recognition by the Saccharomyces cerevisiae "TATA" binding protein promoter-dependent differences in the thermodynamics and kinetics of binding. Biochemistry 37 (1998) 15842-9. 

Five, genetically distinct, /8-D-fructofuranosidases have been described for strains of Saccharomyces hybrids. The behavior and kinetics of each enzyme are very similar.362,451 W. L. Smith and Ballou have purified the mannan-protein /3-D-fructofiiranosidases of three strains of Saccharomyces cerevisiae whose cell walls have differences in mannan structure.452 By use of immunochemical methods, they found that the structure of each /3-D-fructofuranosidase mannan is similar to that of the cell wall of the corresponding strain only. Mutations affecting the structure of the one also produced similar changes in the other. 

Shimoda M, Cocunubo-Castellanos J, Kago H, Miyake M, Osajima Y, Hayakawa I. The influence of CO2 concentration on the death kinetics of Saccharomyces cerevisiae. J Appl Microbiol 2001 91 306-311. 

Proposed Pathway for Formation of Dimethyl Selenide from Selenite in Animals 3-6. Activation and Reduction of Selenate to Selenite in Yeast Saccharomyces cerevisiae 3-7. Conceptual Representation of a Physiologically Based Pharmacokinetic (PBPK) Model for a Hypothetical Chemical Substance 3-8. Selenite Model, a Kinetic Model for Selenite Metabolism 3-9. Selenomethionine Model, a Kinetic Model for Selenomethionine Metabolism 3-10.Existing Information on Health Effects of Selenium 6-1. Frequency of NPL Sites with Selenium Contamination... 

Figure 16.1. Kinetics of H/D exchange of the C2-H in pyruvate decarboxylase from Saccharomyces cerevisiae. The H NMR spectra are expansions showing the ThDP signals C2-H at 9.55 ppm and C6 -H at 7.85 ppm as a nonexchanging standard for quantification.

Donsi, G., Ferrari, G., and Pataro, G. 2007. Inactivation kinetics of Saccharomyces cerevisiae by pulsed electric fields in a batch treatment chamber The effect of electric field unevenness and initial cell concentration. Journal of Food Engineering 78 HA-192. 

Yeast display uses the a-agglutinin receptor to display recombinant proteins on the surface of Saccharomyces cerevisiae. Yeast display of scFvs or Fabs allows the detection and selection by fluorescence-assisted flow cytometry or by magnetic sorting. In addition, flow cytometry can be used for kinetic characterization of antibody affinity (K ) as well as K ff and rates. ... 

Kinetic parameters for the two half reactions are reported in Table 3. It has to be pointed out that there is some confusion in the literature regarding the enantiomeric purity of the AOP samples used in the assays. Mann et al. used racemic AOP, but they have shown unambiguously that only (5)-AOP is the substrate of DAPA AT. (Both enantiomers support the growth of Saccharomyces cerevisiae, but it is probably due to the racemization of (i )-AOP in vivo.) It is claimed in the other studies that the AOP samples used as substrates had the natural 8-(5) configuration but they are very likely also racemic compounds, since they have been obtained under racemizing conditions,as discussed by Mann et Only in the study performed with the... 

Abstract A commercial strain of Saccharomyces cerevisiae was used for the production of ethanol by fermentation of cashew apple juice. Growth kinetics and ethanol productivity were calculated for batch fermentation with different initial sugar (glucose + fructose) concentrations. Maximal ethanol, cell, and glycerol concentrations were obtained when... 

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