Antibody affinity

Pedersen, M.K., Sorensen, N.S., Heegaard, P.M., Beyer, N.H., and Bruun, L. (2006) Effect of different hapten-carrier conjugation ratios and molecular orientations on antibody affinity against a peptide antigen./. Immunol. Meth. 311(1-2), 198-206. 

Many precolumns and trap cartridges for sample clean-up are commercially available. In our experience, a 2 to 3 cm short column with twice the analytical column inner diameter and packed with the same particles performs satisfactorily. An antibody affinity column for selective removal of highly abundant proteins from human serum samples provides better sensitivity for the discovery of low abundance protein markers that may represent revolutionary therapeutic diagnosis and monitoring. 

Antibody affinity from the Latin, affinis = connected with, having things in common. In immunohistochemistry, antibody affinity determines the strength of binding of a monovalent antibody, such as Fab fragment, to one epitope, i.e., how tightly an antibody binds to its particular antigen. 

Secondary antibody affinity-purified fluorescent antiglobulin conjugate reactive with the species globulin of the first step, e.g., affinity-purified rhodamine-con-jugated goat antimouse IgG(H + L chains) (Jackson ImmunoResearch, West Grove, PA), diluted to 25 pg/mL in BSA-PBS. Can be stored at-70°C and reused (see Notes 3 and 4). 

Figure 6.6 Hypothetical comparison of ambient analyte and mass-sensing analysis of thyroid-stimulating hormone. Solid line indicates mass assay dashed line indicates ambient analyte assay. Antibody affinity of 10"i° liter per mole and volume of 100 pL are assumed. Mass assay assumes 10i° binding sites per 100 pL. (From Silzel, J.W. et al., Clin. Chem., 44, 2036-2043, 1998. With permission.)...

Antibodies Affinity labeling of antibody combining sites as illustrated by anti-dinitrophenyl antibodies, 46, 479 p-azoben-zenearsonate antibody, 46, 492 affinity cross-linking of heavy and light chains, 46, 501 bivalent affinity labeling haptens in the formation of model immune complexes, 46, 505 DNP-based diazoketones and azides, 46, 508 labeling of antilactose antibody, 46, 516. 

Steward, M. W. Steensgaard, J. "Antibody Affinity Thermodynamic Aspects and Biological Significance", CRC Press Inc. Boca Raton, Florida, 1983. 

NHH Heegaard. Determination of antigen—antibody affinity by immuno-cap-illary electrophoresis. J Chromatogr A 680 405-412, 1994. 

The determination of antibody affinity (in M 1) is useful for comparison with other reported antibodies. However, the important test of affinity is how well the antibody performs. If more than one antibody has been prepared, a direct comparison of antigen binding curves will indicate the most avid preparation. 

Antibody affinity. The affinity of the antibodies is much greater in the secondary response as opposed to the primary response, which is termed affinity maturation. ... 

Beall, J.A. and Mitchell, G.F. (1 986) Identification of a particular antigen from a parasite cDNA library using antibodies affinity purified from selected portions of Western blots. The Journal of Immunological... 

Somatic Hypermutation in Clonally Related B Cell Products Changes in Antibody Affinity... 

Sharon, J. (1990). Structural correlates of high antibody affinity three engineered amino acid substitutions can increase the affinity of an anti-p-azophenylarsonate antibody 200-fold. Proc. Natl. Acad. Sci. USA 87,4814-4817. 

Antibody affinity chromatography is employed to isolate antigen-specific antibodies. The most common affinity matrix for coupling of molecules is cyanogen bromide-activated... 

Kaufman, E.N. and Jain, R.K. (1992) Effect of bivalent interaction upon apparent antibody affinity experimental confirmation of theory using fluorescence photobleaching and implications for antibody binding assays. Cancer Res., 52,4157 1167. 

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