S. typhimurium

Salmonella heidelberg S. montevideo S. typhimurium S. enteritidis Sarcina lutea Serratia marcescens... 

Thiamine was biosynthesized by resting cells of S. typhimurium strain thilO/T-ath-383, which can synthesize thiamine from exogenous glucose, AIRs, and thiazole.54 Derepression was achieved by conventional means. The organism was cultivated in the presence of a suboptimal amount of thiamine (20 nM), the washed cells were resuspended in a minimal medium containing glucose (10 mM), thiazole (1-2 mM), and labeled AIRs (10 p,M). During the incubation (1.5 hours 37°C), the level of thiamine diphosphate in the cells had risen from about 0.04 to 0.5 nmol/mg. In work with molecules labeled with stable isotopes, thiamine was extracted and cleaved by ethanethiol to 4-amino-5-(ethyl-... 

Scheme 30.—Correspondence between ribose and pyramine carbon atoms in the biosynthesis by S. typhimurium.

Scheme 31.—Correspondence between the nitrogen atoms of AIRs and those of pyramine in S. typhimurium.

S. cerevisiae (D3)/plate incorporation S. cerevisiae (D7)/spot test S. typhimurium Mammalian cells ... 

S. typhimurium (unstabilized TCE, vapor assay) Gene mutation No data McGregor et al. 1989... 

S. typhimurium TAIOO (reverse mutation) Gene mutation Waskell 1978... 

S. typhimurium normally transport trehalose via the galactose permease and are able to grow on this substrate in the complete absence of PTS phosphorylating activity. However, in S. typhimurium which lack a functional galactose permease, IfMan appears to be able to transport trehalose [78]. There is no evidence that trehalose is phosphorylated in this process, again pointing to Il "-dependent transport in the facilitated diffusion mode. 

The fructose-specific PTS in R. sphaeroides is simpler than the one in E. coli or S. typhimurium in that it consists of only two proteins. Besides the fructose specific ll , a class II enzyme, there is only one cytoplasmic component called soluble factor (SF) [48]. We now know that SF consists of IIl , HPr and E-I covalently linked [109]. 11 and SF form a membrane-bound complex whose association-dissociation dynamics is much slower than the turnover of the system. Therefore, the complex is the actual catalytic unit in the overall reaction and P-enolpyruvate is the direct phosphoryl group donor [102],... 

Some toxicity studies were performed analyzing Bfx and Fx mutagenic effects [240-242], For example, Bfx and Bfz nitro-substituted were tested for mutagenicity in Salmonella typhimurium (S. typhimurium) TA 98 and TA 100 strains with and without metabolic activation (Ames test). All the Bfx (10/10) and some of the Bfz (9/15) are mutagenic without activation. Other study has demonstrated benzofuroxan (128, Fig. 20) is mutagenic in the Luria and Del-brueck s fluctuation test, with Klebsiella pneumoniae, and in the Ames test. In another study it has been found that compound 137 (Fig. 21) is not mutagenic to S. typhimurium. 

S. typhimurium (Ames assay) Gene mutation - - Ciba-Geigy 1978a Reofos 95... 

Fe2+ is also transported by the CorA protein (Hantke, 1997), a divalent cation transporter for Mg2+, Co2+, Mn2+, and Ni2+ in E. coli and S. typhimurium. CorA may represent the often-mentioned low-affinity iron-uptake system of E. coli that is suppressed in Fe3+ uptake studies by the addition of 0.2 mM dipyridyl and 0.1 mM nitrilotriacetate. 

Mutagenicity of tetrahydroepoxides. The mutagenic activity, without metabolic activation, of fourteen PAH tetrahydroepoxides has been measured in S. typhimurium strain TA 100. The logarithms of the relative mutagenic activities are plotted in Figure 3 vs. the value of AEj / calculated for conversion of each tetrahydro-... 

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