Rotating wall vessel

Taxus cuspidata rotating wall vessel laminar biomass and metabolite productivity growth [106]... 

Soule et al. [141] constructed a sparged, concentric cylinder bioreactor for the cultivation of suspensions of Pirus malus. Growth was reduced under all rotational conditions. Sun and Linden [106] employed a rotating wall vessel (Rotary Cell Culture System, Synthecon, Houston, TX, USA) to cultivate suspensions of Taxus cuspidata under laminar flow conditions. Shear rates were... 

Rhee HW, Zhau HE, Pathak S et al (2001) Permanent phenotypic and genotypic changes of prostate cancer cells cultured in a three-dimensional rotating-wall vessel. In Vitro Cell Dev Biol Anim 37 127-140... 

Becker JL, Prewett TL, Spaulding GF Goodwin TJ (1993) Three-dimensional growth and differentiation of ovarian tumour cell lines in high aspect rotating wall vessel. Morphologic and embryonic considerations. Journal of Cellular Biochemistry 51 283. 

Goodwin TJ, Jessup JM Wolf DA (1992) Morphological differentiation of human colorectal carcinoma in rotating wall vessels. In Vitro Cell Development Biology 28A 47-60. 

Goodwin TJ, Schroeder WF, Wolf DA Moyer MP (1993) Rotating-wall vessel coculture of small intestine as a prelude to tissue modeling aspects of simulated microgravity. Proceedings of Society for Experimental Biology and Medicine 202 181-192. 

Sikavitsas, V.I., Bancroft, G.N., and Mikos, A.G., Formation of three-dimensional ceU/polymer constructs for bone tissue engineering in a spinner flask and a rotating wall vessel bioreactor. J. Biomed. Mater. Res., 2002,62 136-148. 

Most Common Bioreactors in Tissue Engineering— 44-2 Spinner Flask Rotating-Wall Vessels Perfusion... 

Bone is a hard connective tissue that provides mechanical support to the human body and is a frame for locomotion. Bone grafts have been generated under a wide variety of culturing conditions, including static and dynamic systems. Among the most popular dynamic systems are spinner flasks, rotating-wall vessels. 

The effect of shear stress has also been conducted in a rotating-wall vessel [11]. Variation of the rotation speed of the mobile wall when culturing chondrocytes in poly(lactic acid) (PLA) foams for 28 days did not cause a change in cell density among scaffolds cultured at different rotation rates although they were... 

FIGURE 15 Design of a rotating wall vessel bioreactor. Cells are suspended in medium, which fills the vessel until no air bubble is left. Oxygen is delivered via a silicone membrane in the center of the vessel. The vessel rotates at a relatively low speed ( 30 rotations/min) to prevent settling of cells. 

Spinner Flask, and Rotating Wall Vessel Bioreactors Perfusion Bioreactors Mechanical Loading Bioreactors Surface Shear Bioreactor Limitations and Challenges... 

Schwarz, R. P., T. J. Goodwin et al. 1992. Cell culture for three-dimensional modeling in rotating-wall vessels An application of simulated microgravity. / Tissue Culture Methods 14 51-58. 

Licato LL, Prieto VG, Grimm EA. A novel precUnical model of human maUgnant melanoma utilizing bioreactor rotating-wall vessels. In Vitro Cell Dev Biol Anim 2001 37 121-6. 

Bioreactor Rotating Wall Vessel Perfusion Bioreactors... 

FIGURE 38.1 Schematic representation of several bioreactor configurations used for stem cell culture, (a) Spinner flask (b) stirred suspension bioreactor (c) wave bioreactor and (d) rotating wall vessel. The slow turning lateral vessel (STLV) configuration is shown (e) hollow-fiber bioreactor and (f) packed-bed bioreactor. 

A viable alternative approach for undifferentiated MSC culture was recently described where cells are cultured as 3D aggregates or spheroids (Bartosh et al., 2010 Frith et al., 2010). Since it is believed that this approach may lead to an increase of the MSC therapeutic potential (Bartosh et al., 2010), methods were developed for dynamic 3D in vitro MSC culture using spinner flasks and rotating wall vessel bioreactors (Frith et al., 2010). Altered cell size and surface antigen expression, together with enhanced... 

Duke J, Daane E, Arizpe J, Montufar-Solis D. 1996. Chondrogenesis in aggregates of embryonic Umb cells grown in a rotating wall vessel. Adv Space Res 17(6-7) 289-93. 

Liu Y, Liu T, Fan X, Ma X, Cui Z. 2006. Ex vivo expansion of hematopoietic stem cells derived from umbilical cord blood in rotating wall vessel./Biotedi 124(3) 592-601. 

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