ROSETTA method

Rohl, C. A., C. E. Strauss, K. M. Misura, and D. Baker. 2004. Protein structure prediction using Rosetta. Methods Enzymol 383 66-93. 

In particular, the known stress calibration method was chosen, therefore 6 rosetta strain gauges (R1-R6) on the shell and 7 (R7-R13) on the the head were applied. Their distances measured from the head centre are listed in table 1. R3 and R4 were applied only to check a uniform stress level on the shell surface. 

Figure 6.2. Domain fusion method to detect functional linkages between proteins. If two proteins, A and B, from a certain species are expressed as a single fused protein within another species, the proteins A and B are proposed to be functionally linked. Because the fusion protein contains homology to both A and B, it is termed a Rosetta Stone sequence. Specific examples from domain fusion analyses are shown. Figure adapted from Eisenberg et al. (2000) and Marcotte et al. (1999).

We might think that the Murchison meteorite would have been studied thoroughly enough in the years since its arrival on Earth. But the results obtained always depend on the performance of the technical resources available in this case, the analytical methods and the apparatus. Thus, it is not really surprising that a new class of amino acid has been discovered in Murchison material diamino acids, such as DL-2,3-diaminopropionic acid, DL-2,4-diaminobutanoic acid etc. These were identified using a new enantioselective GLPC/MS method, which is also being used in analysis of material from the Rosetta mission. 

Written records provide a valuable glimpse into the thoughts and behavior of ancient peoples. But these records are not complete and omit important information (important, that is, to historians). Scientists studying the past have had to turn to other sources such as analyzing artifacts. Archaeological finds are a rich source of information, if that information can be unlocked. The need is to find a Rosetta stone for other types of artifacts—a method of gleaning all the information that is available. 

In accordance with the focus of this chapter on current proteins structure prediction methods, and despite some considerable improvements of ah initio methods (e.g. the fragment assembly approach ROSETTA [37],... 

Recently, the Rosetta stone method has been introduced. This method uses over 20 completely sequenced genomes and analyzes evolutionary correlations of two domains being fused into one protein in one species and occurring in separate proteins in another species. From these classifications the method establishes pairwise links between functionally related proteins [94] and elicits putative protein-protein interactions [95]. 

Rosetta stone method If two proteins occur separated in one organism and homologous proteins are fused in another organism, the former two proteins are candidates for being binding partners in the first organism [31]. 

Gene Fusions The Rosetta Stone Method STRING (http //string.embl.de/)... 

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