Putative receptor proteins

Oelrichs, R.B. Reid, H.H. Bernard, O. Ziemiecki, A. WRks, A.F. NYK/ FLK-1 a putative receptor protein tyrosine kinase isolated from ElO embryonic neuroepithelium is expressed in endothelial cells of the developing embryo. Oncogene, 8, 11-18 (1993)... 

Ronsin, C. Muscatelli, F. Mattel, M.G. Breathnach, R. A novel putative receptor protein tyrosine kinase of the met family. Oncogene, 8, 1195-1202 (1993)... 

Apelin, human apelin LVQPRGSRNG PGPWQGGRRK FRRQRPRLSH KGP MPF, a 36-peptide acting as the endogenous ligand of the orphan G protein-coupled receptor APJ (putative receptor protein related to the angiotensin II receptor ATi). APJ consists of 377 aa and... 

The receptor for influenza C is assumed to be 9-0-acetyl sialic acid, based on the esterase activity of its receptor-destroying enzyme [10]. In vivo, 9-O-acetyl sialic acids are added selectively to glycoproteins and glycolipids in a limited range of cell types, although these cell types are reasonably widely distributed [11], including mucosal epithelia. A putative receptor protein, a 40 kDa mucin heavily modified by 9-O-acetyl sialic acid, is present on apical surfaces of permissive MDCK cells and is rapidly and constitutively endocytosed [12],... 

An alternative approach to clone the receptor was taken by Thomas and colleagues (1990, 1991). They screened a cDNA library from peritoneal rabbit cells with an antisense oligonucleotide probe deduced from the coding region of the second transmembrane domain, which is common to G-protein-coupled receptors (fMet-Leu-Phe is a G-protein-linked receptor). Screening of this library yielded a putative receptor clone F3R, characterised as follows ... 

MacLennan, A.J., Browe, C.S., Gaskin, A.A., Lado, D.C. and Sbaw, G., 1994, Cloning and cbaracterization of a putative G-protein coupled receptor potentially involved in development. Mol. Cell. Neurosci. 5 201-209. 

Okazaki, H., Ishizaka, N., Sakurai, T., Kurokawa, K.., Goto, K., Kumada, M. and Takuwa, Y., 1993, Molecular cloning of a novel putative G protein-coupled receptor expressed in the cardiovascular system, Bioc/jem. Biophys. Res. Comm. 190 1104-1109. 

When screening the protein library for putative receptors of MRS-2179, the P2Yi receptor is indeed ranked among the top scorers (7th, Fig. 12A). 

YH Chen, Y Xiao, W Wu, Q Wang, G Luon, MP Dierich. HIV-2 transmembrane protein gp36 binds to the putative cellular receptor proteins P45 and P62. Immunobiology 201 317-322, 2000. 

McCormack DG, Clarke B, Barnes PJ (1989) Characterization of adenosine receptors in human pulmonary arteries. Am J Physiol 256(2) H41-H46 McWhinney CD, Dudley MW, Bowlin TL, Peet NP, Schook L, Bradshaw M, De M, Borcherding DR, Edwards CK 3rd (1996) Activation of adenosine A3 receptors on macrophages inhibits tumor necrosis factor-alpha. Eur J Pharmacol 310(2-3) 209-216 Mentzer RM Jr, Rubio R, Berne RM (1975) Release of adenosine by hypoxic canine lung tissue and its possible role in pulmonary circulation. Am J Physiol 229(6) 1625-1631 Meyerhof W, Mtiller-Brechlin R, Richter D (1991) Molecular cloning of a novel putative G-protein coupled receptor expressed during rat spermiogenesis. FEBS Lett 284(2) 155-160... 

Fig. 4. Receptor contact sites on the G protein. Ribbon diagram of the heterotri-meric G protein (1GOT) with the C-terminal tail of Gat attached (1AQG), which has been rotated toward the viewer from the putative receptor-binding orientation to visualize the receptor contact sites (magenta).

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