Roots, protein extraction from

Partial Purification of SA-GTase. Proteins extracted from oat roots Incubated for 20 h in salicylic acid were separated by salt precipitation and gel exclusion and anion exchange chromatography (Table IV). A 5 -fold purification of the SA-GTase was achieved with this... 

Protein extraction from control and allelochemical-treated roots of target... 

Enzyme assays For enzyme activities, total protein is extracted from seedlings of controls and after 72 h-treatment under native conditions. Primary roots are homogenized in extraction buffer (for formulation see... 

The activity and total protein extracted may vary according to the extraction procedure and medium used. The buffer-to-tissue ratio was an important factor in the extraction of PPO from sweet potato root. PPO was extracted using ratios of 2-6 1 v/m and the highest specific activity was obtained at a ratio of 4 1 v/m [43]. It was also found that PPO could be extracted with a phosphate buffer solution of low... 

The synthesis of BOA-6-O-glucoside is catalyzed by constitutive enzymes that may be upregulated. A glucosyltransferase that accepted BOA-6-OH as a substrate was measurable in protein extracts of corn roots harvested from control plants. Detoxification via glucoside carbamate synthesis is inducible and seems to be more complicated than simple A/-glucosylation (data unpublished). The biosynthesis of the compounds is part of our ongoing research. 

D-PAGE of cytoplasmic proteins from bean and tomato roots (A) bean control (B) bean treated with A. sedillense aqueous extract (C) tomato control (D) tomato treated with A. sedillense aqueous extract (From Romero-Romero, M. T. et al. 2002, J. Chem. Ecol. 28, 601-613. With permission). 

D-PAGE of cytoplasmic root proteins of tomato. (A) control (B) treated with C. acuminata aqueous extract (C) protein 5 was N-terminal microsequenced, comparison is shown in panel (From Cruz-Ortega, R. et al. 2002, Physiol. Plantarum, 116, 20-27. With permission.)... 

Plant tissue collection for DNA and protein studies has primarily utilized leaf tissue, but seeds, roots, flowers, stems, pollen, spores, and game-tophytes have all been used successfully. For example, DNA extraction from the parasitic Cuscuta required using only intemode tissue to prevent DNA contamination from its host species26 likewise, the green stems of a leafless Koeberlinia provided an adequate source of DNA.27... 

A number of species that are known to be or may be present in root nodules can oxidize Fe(II) Lb. These species include nitrite (92), superoxide (93), and peroxides (94). The process would be expected to be relatively facile, as the reduction potential for the Fe(III) Lb/Fe(II) Lb couple has been reported to be +0.22 V at pH 7 and +0.27 V at pH 6 (95). Even in cases in which considerable care has been taken during the extraction process, most extracts from root nodules contain at least traces of the Fe(III) form of the protein. Whether this arises... 

Protein tyrosine kinases are enzymes that catalyze the transfer of phosphate from adenosine triphosphate (ATP) to the hydroxyl group of the amino acid tyrosine on many essential proteins. These proteins play an essential role in cell signalling, regulation of cell growth and transformation of the cells [10]. The identification of specific inhibitors of protein tyrosine kinase may uncover potential anticancer agents [10]. Jayatilak et al. [60] investigated the kinase inhibitor activity of stilbene compounds, 15-20, that were extracted from the polar methanol fraction of Polygonum cuspidatum roots. The compounds were evaluated in protein... 

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