DNA hybridization detection

Results from a DNA-hybridization detection achieved with FEDs differ in ... 

T.-Y. Lee and Y.-B. Shim, Direct DNA hybridization detection based on the oligonucleotide-functionahzed conductive polymer. Ainal. Chem. 73, 5629—5632 (2001). 

Jiang G, Susha AS, Lutich AA et al (2009) Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection. ACS Nano 3 4127—4131... 

Gaylord BS, Heeger AJ, Bazan GC (2003) DNA hybridization detection with water-soluble conjugated polymers and chromophore-labeled single-stranded DNA. J Am Chem Soc 125 896-900... 

Liu B, Bazan GC (2007) Energy transfer between a cationic conjugated poly(lluorene-co-phenylene) and thiazole orange for DNA hybridization detection involving G-rich sequences. Macromol Rapid Commun 28 1804—1808... 

Cai H, Cao XN, Jiang Y, He PG, Fang YZ (2003) Carbon nanotube-enhanced electrochemical DNA biosensor for DNA hybridization detection. Anal. Bioanal. Chem. 375 287-293. 

Explain how surface chemistry and coverage affect the success of electrical DNA-hybridization detection schemes. 

Fig. 5 Immobilized nucleic acid assays utilizing redox-active moieties, a Amplified detection of viral DNA by generation of a redox-active replica and the bioelectrocatalyzed oxidation of glucose (Reprinted with permission from [200]. Copyright(2002) American Chemical Society), b Alternative formats for the capture on a gold electrode SAM of solution-extended primers or direct surface extension of primer with electrotides (adapted from [185]). c Ferrocene-labelled hairpin for electrochemical DNA hybridization detection. A Fc-hairpin-SH macromolecule is immobilized on a gold electrode. When a complementary DNA target strand binds to the hairpin, it opens and the ferrocene redox probe is separated from the electrode, producing a decrease in the observed current (Reprinted with permission from [203], Copyright(2004) American Chemical Society)...

Kim, C.K., Kallum, R.R., Singh, J.P., Fortner, A., Griffin, J., Darbha, G.K., and Ray, PC. 2006. Gold nanoparticle based miniaturized laser induced fluorescence probe for specific DNA hybridization detection Studies on size dependent optical properties. Nanotechnology, 17 3085-93. 

Chitosan has been utilized along with hydrothermally prepared nano-ZnO nanoparticles for DNA hybridization detection. The detection limit is obtained as 1.09 x 10"11 mol L 1 of complementary target [66], A MWNTs/nanoZnO/CH modified nanocomposite GCE electrode has been used for DNA immobilization via physisorption. This biosensor can effectively discriminate different DNA sequences related to PAT gene in the transgenic com, with detection limit of 2.8 x K)"12 mol/L of target sequence [67], In another report they have deposited ZnO nanoparticles, MWNTs and CH layer onto glassy carbon electrode to immobilize ssDNA probe. A remarkable synergistic effect of the ZnO nanoparticles and MWNTs has been achieved after ssDNA probe immobilization for fabrication of sensitive electrochemical DNA biosensor. The modified electrode shows a wide linear response for DNA hybridization detection. (1.0 x 10"11 to 1.0 x 10"6 mol/L) with detection limit of 2.8 x 1 O 12 mol/L [68],... 

Archer M, Christophersen M, Fauchet PM (2004) Macroporous silicon electrical sensor for DNA hybridization detection. Biomed Microdevices 6 203-211... 

Zhang Q, Subramanian V (2007) DNA hybridization detection with organic thin film transistors toward fast and disposable DNA microarray chips. Biosens Bioelectron 22 3182-3187... 

Wang et al [24] first reported a DNA hybridization detection method based on the precipitation of silver on Au-NP tags and subsequent electrochemical stripping detection of the dissolved silver. The assay employed a sandwich-like protocol with streptavidin-Au-NPs labeling the biotinylated-breast cancer gene (BRCAl) sequences. 

Later on, Lee et al. [26] reported the electrocatalj ic effect of Au-NPs on silver electrodeposition upon ITO-based electrodes (Fig. 5.3), in absence of pre-oxidation steps, and its successful application to the DNA hybridization detection obtaining a signal-to-noise ratio of 20 that presented a great improvement in relation to their previous works under similar conditions. 

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