Rolling circle

Kuhn H., Demidov V.V., Frank-Kame-NETSKii M.D. Rolling-circle amplification under topological constraints. Nucleic Acids Res. 2002 30 574-580... 

Regression correlation coefficient Regression coefficient of determination Rolling circle amplification Water solubility Sodium dodecyl sulfate Supercritical fluid extraction Standard operating procedure Solid-phase extraction Surface plasmon resonance Thymine... 

Many nucleic acid detection strategies use target amplification, signal amplification or both. Invader, branched DNA (bDNA) and rolling circle amplification (RCA) are three approaches. 

Figure 5.14 Events in the replication of < >X174 DNA. (a) Formation of the replicating double-stranded DNA (RF). (b) Rolling circle replication leads to the formation of virus progeny.

Rolling circle amplification (RCA) is an alternative method to polymerase chain reaction it is also a generic amplification technique that can be used in antibody assays. Using a replication process similar to that used by viruses, RCA allows the recognition,... 

Since rolling circle amplification takes place at a constant temperature, there is no need for the target amplification process to take place in a thermal cycler, which is required to regulate the temperature for different parts of the reaction. The type of DNA polymerase to be used in RCA is not limited to thermostable enzymes, like the PCR-based diagnostics. On the other hand, the RCA method requires the environment to be free of contaminations as the RCA arrays are highly sensitive. Wiltshire [22]... 

D.C. Thomas, G.A. Nardone, and S.K. Randall, Amplification of padlock probes for DNA diagnostics by cascade rolling circle amplification or the polymerase chain reaction. Arch. Pathol. Lab. Med. 123, 1170-1176 (1999). 

S. Wiltshire, S. O Malley, J. Lambert, K. Kukanski, D. Edgar, S.F. Kingsmore, and B. Schweitzer, Detection of multiple allergen-specific IgEs on microarrays by immunoassay with rolling circle amplification. Clin. Chem. 46,1990-1993 (2000). 

Fig. 4.12 The structural relationship between graphene sheet and single-walled carbon nanotube arrows point to two alternative directions of rolling circles inside rings reflect on aromatic character of carbon rings and delocalization of resonant electrons inside them...

Figure 1.16 Critical issues facing DNA microarrays and protein microarrays. MIAME = minimum information about a microarray experiment, checklist endorsement as of October 14, 2002. RCA = rolling circle amplification. RLS = resonance light scattering.

Note ELF = enzyme labeled fluorescence. ECL = enhanced chemiluninescence. CL = chemiluminescence. RES = biotin amplification, sAV-Cy3. RCA = rolling circle amplification. 

It is apparent that signal amplification provides increased sensitivity over direct labeling. This is especially true for fluorescent-based assays. One of the most sensitive signal detection technologies is the immunoRCA (Schweitzer et al., 2000). Rolling circle amplificahon (RCA) is combined with antibody detection. RCA involves the amplification of circularized oligonuceotide probes under isothermal conditions by DNA polymerase (Lizardi et al., 1998). With immunoRCA, the 5 primer is attached to the reporter antibody. Initiation of the amplification starts when circular DNA template binds to the attached primer. 

Lizardi, P.M., Huang, H., Zhu, Z., Bray-Ward, P, Thomas, D.C., and Ward, D.C., Mutation detection and single-molecule counting using isothermal rolling circle amplification, Nat. Genetics, 19, 225-232, 1998. 

Schweitzer, B., Wiltshire, S., Lamber, J., O Malley, S., Kukanskis, K., Zhu, Z., Kingsmore, S.E, Lizardi, F.M., and Ward, D.C., Immunoassays with rolling circle DNA amplification a versatile platform for ultrasensitive antigen detection, Proc. Natl. Acad. Sci. USA, 97, 10113-10119, 2000. 

A novel signal amplification method based on the rolling circle amplification (RCA) technology has also been reported. RCA involves many rounds of isothermal enz5miatic... 

Nallur G, Luo C, Fang L et al. Signal amplification by rolling circle amplification on DNA microarrays. Nucleic Acids Res 2001 29 E118. 

After the formation of the RF, the bacteriophage-encoded gp2 cleaves the positive strand within the intergenic region, which rolls off from the negative strand. During this process, both strands are again replicated to produce double-stranded RFs. This type of replication, known as the rolling circle... 

Rolling-circle replication. A mechanism for the replication of circular DNA. A nick in one strand allows the 3 end to be extended, displacing the strand with the 5 end, which is also replicated, to generate a double-stranded tail that can become larger than the unit size of the circular DNA. 

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