Rhodamine B, reagent

Spray solution Dissolve 10 to 500 mg rhodamine B in 100 ml ethanol [3, 7], methanol [5] or water [2], 

Storage Both reagent solutions may be stored over a long period. 

Because it contains amino and carboxylic groups rhodamine B tends to form zwitter ions which easily associate and can accumulate in lipophilic chromatogram zones. 

The chromatograms are freed from mobile phase and immersed in the reagent solution for 1 s or evenly sprayed with it. Red-violet zones are usually formed on a pink background they fluoresce a deeper red than their surroundings in long-wavelength UV light (A = 365 nm). 

Note Rhodamine B is a universal reagent that can be used on silica gel, talc, starch [5] and cellulose layers, just as on urea [1] or silver nitrate-impregnated [7] phases. Liquid paraffin-impregnated silica gel and RP layers are less suitable, since the background to the chromatographic zones is also intensely colored. It is often possible to increase the detection sensitivity by placing the plate in an atmosphere of ammonia after it has been sprayed or dipped, alternatively it can be oversprayed with sodium or potassium hydroxide solution. 

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Another sensitive colorimetric procedure is that of Mackenzie et al (1967), which utilizes the dye Rhodamine B to form benzene-soluble complexes with fatty acids. Nakai et al. (1970) developed a rapid, simple method for screening rancid milk based on the foregoing procedure. The test is said to detect rancid milk with an ADV above 1.2. Like the copper or cobalt soap method, the Rhodamine B reagent is also limited to the longer-chain fatty acids. Kason et al (1972) used the method employing Rhodamine 6G of Chakrabarty et al (1969) to investigate... 

Rhodamine-B reagent see antimony(III) compounds (Section III.15, reaction 7). There is no need for preliminary oxidation in this case. 

Or - To 2 drops of the solution on a spot plate, add a minute crystal of NaN02, stir, and add 2 drops of Rhodamine-B reagent. 

Stannic chloride (Rgt. No. 236) is now only rarely used for detection. If no success is obtained with fluorescence layers, iodine vapour (Rgt. No. 141) or rhodamine B reagent (No. 220) can be used for non-destructive detection. 

A layer impregnated with fluorescein (Rgt. No. 121,6) has been used for detection. Pink spots are formed by amounts exceeding 5 (xg after standing for 1—2 h. Dark blue-violet spots on the green fluorescent layer are seen in long-wave UV radiation. The rhodamine B reagent (No. 120) can also be used, followed by spraying with a 10 % solution of sodium carbonate [101] (see also Rgt. No. 77). 

Rhodamine B 67 is frequently used in the quantitative determination of DNA or RNA and fluorescent labeling for DNA [190-192]. This dye was assembled onto the surface of a quartz substrate by electrostatic interaction between the fluorescence reagent RB and y-aminopropyltriethoxysilane (APES), and the Quartz/ APES/RB film was constructed (Fig. 1) [193]. 

Lissamine rhodamine B sulfonyl hydrazine is soluble in DMF. The reagent may be dissolved in this solvent as a concentrated stock solution before adding a small aliquot to an aqueous reaction medium. The compound itself and all solutions made with it should be protected from light to avoid decomposition of its fluorescent properties. 

Lissamin rhodamine B sulfonyl chloride chemiluminescence reagent, 5 852-853 Lister, Joseph, 11 7 Literature research, in fine chemical... 

Analysis. Colorimetric analysis of Ga permits detection of 20 ppb. Ga or a Ga compound in the flame gives a violet (lilac) color. This is due to its emission of 414.0 nm photons. This photon line allows for the spectrophotometric determination of Ga by AAS, which is sensitive to about 10 ppm. ETAAS increases this sensitivity to 5 ppb, and ICPAES to about 1 ppb, with ICPMS going as low as 0.1 ppb. Rhodamine B is a reagent which facilitates the spottest detection at a level of 10 ppm or above. 

Specific reagents react with a particular class of compound, For example, rhodamine B is often used for visualization of lipids, ninhydrin for amino acids, and aniline phthalate for carbohydrates. 

Generalized protocols for the use of hydrazine-probes reactive toward aldehyde residues can be found in Section 1.1. These procedures are directed at the labeling of cell surface glycoproteins or glycoproteins in solution. Substitution of Lissamine rhodamine B sulfonyl hydrazine for the fluorescein-5 -thiosemicarbazide reagent described in that section can be done without difficulty. Some optimization may be... 

On the other hand, Bersis and Vassiliou [5] have attempted to use gallic acid as an ozone acceptor and rhodamine-B as a photon emitter that remains unchanged during the measurement. They used an ethanol solution containing gallic acid and rhodamine-B as reactive reagents, which meant that this method could not be applied to a solid sensor. 

Color forming reagent, Rhodamine B soln. should have a strength of 0.01% in 0.5 ATHC1. 

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