Rhizobium mutants

Construction of a broad host range cloning vector and its use in the genetic anlysis of Rhizobium mutants. Gene 18 289-296... 

Cock, J.M., Mould, R.M., Bennett, M.J. Cullimore, J.V. (1990). Expression of glutamine synthetase genes in roots and nodules of Phaseolus vulgaris following changes in the ammonium supply and infection with various Rhizobium mutants. Plant Molecular Biology 14, 549-60. 

Mutant YE-2 of Rhizobium meliloti excretes a mixture of soluble polysaccharides that include a complex succinoglycan having a branched octasaccharide repeat as well as a simple galactoglucan (22) having a linear disaccharide repeat.102 In contrast to the case of the succinoglycan, oriented fibers of the potassium salt of 22 have yielded good X-ray data and its three-dimensional structure has been established.39 The polymer forms a two-fold helix of pitch... 

Rhizobium have been reported, but to date there is little evidence that siderophores produced by pseudomonads are beneficial for promoting nodulation and nitrogen fixation. In experiments examining the role of siderophore production on nodulated clover plants, siderophore-defective mutants were shown to stimulate growth of nodulated clover plants similarly to the siderophore-producing parent strain (119). 

M. Marek-Kozaczuk, M. Deryto. and A. Skorupska, Tn5 insertion mutants of Pseu-domoiuis sp. 267 defective sidcrophore production and their effect on clover (Trifo-liiim pnuense) nodulated with Rhizobium leguminosarum bv. trifolii. Ploul Soil 779 269 (1996). 

Cooper JB, Long SR. 1994. Morphogenetic rescue of Rhizobium meliloti nodulation mutants by trans-zeatin secretion. Plant Cell 6 215-225. 

Murray JD, Karas BJ, Sato S, Tabata S, Amyot L, Szczyglowski K. 2007. A cytokinin perception mutant colonized by Rhizobium in the absence of nodule organogenesis. Science 315 101-104. 

Nadler, K.D. (1981). A mutant strain of Rhizobium leguminosarium with an abnormality in heme biosynthesis. In Current Perspectives in Nitrogen Fixation, ed. A.H. Gibson W.E. Newton, p. 414. Canberra Australian Academy of Science. 

Carlson, R.W., Kalembasa, S., Turowski, D., Pachori, P., Noel, K.D. Characterization of the lipopolysaccharide from a Rhizobium phaseoli mutant that is defective in infection thread development. J Bacteriol 169 (1987) 4923 1928. 

Forsberg, L.S., Noel, K.D., Box, J., Carlson, R.W. Genetic locus and structural characterization of the biochemical defect in the O-antigenic polysaccharide of the symbiotically deficient Rhizobium etli mutant, CE166 Replacement of N-acetylquinovosamine with its hexosyl-4-ulose precursor. J Biol Chem 278 (2003) 51347-51359. 

Ingram, B., Sohlenkamp, C., Geiger, O., Raetz, C.R. Altered lipid A structures and polymyxin hypersensitivity of Rhizobium etli mutants lacking the LpxE and LpxF phosphatases. Biochim Biophys Acta. Biochem. Biophys. Acta 1801 (2010) 593-604. 

Lopez-Lara, I.M., Orgambide, G., Dazzo, F.B., Olivares, J., Toro, N. Surface polysaccharide mutants of Rhizobium sp. (Acacia) strain GRH2 Major requirement of lipopolysaccharide for successful invasion of Acacia nodules and host range determination. Microbiology 141 (1995) 573-581. 

Lerouge, I., Laeremans, T., Verreth, C., Vanderleyden, J., van Soom, C., Tobin, A., Carlson, R.W. Identification of an ABC transporter for export of the O-antigen across the inner membrane in Rhizobium etli based on the genetic, functional and structural analysis of an lps mutant deficient in O-antigen. J Biol Chem 276 (2001) 17190-17198. 

Noel, K.D., Sanchez, A., Fernandez, L., Leemans, J., Cevallos, M.A. Rhizobium phaseoli symbiotic mutants with transposon Tn5 insertions. J Bacteriol 158 (1984) 148-155. 

Perotto, S., Brewin, N.J., Kannenberg, E.L. Cytological evidence for a host defense response that reduces cell and tissue invasion in pea nodules by hpopolysaccharide- defective mutants of Rhizobium leguminosarum strain 3841. Mol Plant-Microbe Interact 7 (1994) 99-112. 

Ridley, B.L., Jeyaretnam, B.S., Carlson, R.W. The type and yield of lipopolysaccharide from sym-biotically deficient Rhizobium hpopolysaccharide mutants vary depending on the extraction method. Glycobiology 10 (2000) 1013-1023. 

Russa, R., Bruneteau, M., Shashkov, A.S., Urbanik-Sypniewska, T., Mayer, H. Characterization of the lipopolysaccharides from Rhizobium meliloti strain 102F51 and its nonnodulating mutant WL113. Arch Microbiol 165 (1996) 26-33. 

VandenBosch, K.A., Noel, K.D., Kaneko, Y., Newcomb, E.H. Nodule initiation elidted by noninfective mutants of Rhizobium phaseoli. J Bacteriol 162 (1985) 950-959. 

Vedam, V., Haynes, J.G., Kannenberg, E.L., Carlson, R.W., Sherrier, D.J. A Rhizobium leguminosarum lipopolysaccharide lipid A mutant induces nitrogen-fixing nodules with delayed and defective bacteroid formation. Mol Plant-Microbe Interact 17 (2004) 283-291. 

New Structures and Stereochemistry.—New Carotenoid Structures. A mutant strain of Rhizobium lupini contains a new nor-carotenoid, 2, 3 -trans-dihydroxy-2-nor-/3,/3-carotene-3,4-dione (1)." The wild-type R. lupini, when cultured in the presence of the cyclization inhibitors nicotine or CPTA, produced three new monocyclic carotenoids, 2,3-h-ans-dihydroxy-/3,-caroten-4-one (2), 3-hydroxy-/3,(/ -caroten-4-one (3), and p,tf/-carotene-2,3-trans-dio (4)," which were characterized by m.s. and n.m.r. The light absorption and mass spectra of a carotenoid from Rhodopseudomonas capsulata allowed its identification" as demethylspheroidenone [l-hydroxy-3,4-didehydro-l,2,7, 8 -tetrahydro- /f,(/f-caroten-2-one (5)]. 

The development of conjugation systems for gene transfer in the fastgrowing strains of Rhizobium has provided a method for studying genetic lesions in symbiotically defective mutants isolated directly from nodules or produced by mutagenic treatments (Maier and Brill, 1978 Beringer et al.. 

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