Broad host range cloning vectors

Construction of a broad host range cloning vector and its use in the genetic anlysis of Rhizobium mutants. Gene 18 289-296... 

Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, Peterson KM. Four new derivatives of the broad-host-range cloning vector pBBRlMCS, carrying different antibiotic-resistance cassettes. Gene 1994 166 800-2. 

Isolation and sequencing of the cellulose synthase gene(s) has not been accomplished yet however, DNA from Acetobacter xylinum containing this gene(s) was cloned into broad host-range plasmid vectors (82). These vectors were mobilized into Pel- mutants to test for complementation. To date, this approach has not produced a pellicle-forming transconjugant from a Pel- mutant of Acetobacter (82). The direct correlation between cellulose production and presence of plasmid DNA in Acetobacter has been reported... 

The genes for both of the lactate dehydrogenase enzymes, l-LDH and d-LDH, that control lactic acid stereospecificity in the lactobacilli have been cloned and sequenced. The l-LDH enzyme from a variety of lactic acid bacteria forms a closely related class entirely distinct from the d-LDH enzymes which form a second closely related group of proteins. With broad host-range integration vectors now available, it is possible to... 

Farinha, M.A. Kropinski, A. M. (1989). Construction of broad-host-range vectors for general cloning and promoter selection in Pseudomonas and Escherichia coli. Gene, 11, 205-10. 

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