Resveratrol stress

Olas and Wachowicz (2002) investigated the effects of tranx-resveratrol and vitamin C on oxidative stress in blood platelets. The level of 02 in control blood platelets and platelets incubated with resveratrol or vitamin C was recorded using a chemiluminescence method. On the other hand, Oh and others (2006) reported the x02 quenching activities of various freshly squeezed fruit and vegetable juices by measuring their inhibitory effects on the rubrene oxidation induced by x02 from disproportionation of hydrogen peroxide by sodium molybdate in a microemulsion system. 

Kasdallah-Grissa, A. et al. (2007) Resveratrol, a red wine polyphenol, attenuates ethanol-induced oxidative stress in rat liver. Life Sciences, 80 (11), 1033-1039. 

Pezet R, Perret C, Jean-Denis JB, Tabacchi R, Gindro K, Viret O. 2003. 8-Viniferin, a resveratrol dihydrodimer One of the major stilbenes synthesized by stressed grapevine leaves. J Agric Food Chem 51 5488-5492. 

It is also well documented that an antioxidant-rich diet protects against age-related macular degeneration (AMD), the leading cause of vision loss among the elderly. Development of AMD in the retinal pigment epithelium (RPE) is associated with oxidative stress. King and co-workers [2005] reported that resveratrol treatment (100 pmol/L) inhibited basal and H202-induced... 

Heiss EH, Schilder YD, Dirsch VM. 2007. Chronic treatment with resveratrol induces redox stress- and ataxia telangiectasia-mutated (ATM)-dependent senescence in p53-positive cancer cells. J Biol Chem 282 26759-26766. 

Langcake P. 1981. Disease resistance of Vitis spp. and the production of the stress metabolites resveratrol, -viniferin, a-viniferin and pterostilbene. Physiol Plant Pathol 18 213-226. 

Modulation of Endothelial Cell Response to Shear Stress by Resveratrol... 

Bruder, J.L., Hsieh, T.C., Lerea, K.M., Olson, S.C., and Wu, J.M., Cell biological and biochemical analysis of induced cytoskeletal changes in bovine artery endothelial cells by resveratrol and the accompanying modified responses to arterial shear stress, BMC Cell Biol, 2, 1, 2001. 

NQOl is a homodimer with a flavodoxin fold (5). This enzyme does not stabilize the semiquinone state. The obligate two-electron transfer mechanism prevents the generation of quinone radicals and redox cycling, which would result in oxidative stress. The NADPH and quinone substrates occupy the same site, consistent with the observed ping-pong bi-bi mechanism. NQOl is inhibited by many (poly)aromatic compounds including the anticoagulant dicoumarol and the phytoalexin resveratrol (5). 

Resveratrol (1) is famous for its antioxidant activities, which could reduce or diminish oxidative stress and provide cardiovascular protection [448-451,494,495]. Recently, the structural basis of antioxidant activity of resveratrol (1) has been evident based on ab initio calculations and experimental crystal structure [496]. 

Therefore, the search for a stimulator of SIRTl was on. One of the first compounds found that is a natural activator of sirtuins is a small molecule called resveratrol, which is present in red wine and made by many plants when stressed. Feeding resveratrol to yeast, worms, or flies, or placing them on a calorie-restricted diet, extends their life spans about 30%, but only if they possess the SIR2 gene. 

Many dietary factors are believed to exert their potent antidisease effects because they possess strong antioxidant activities. However, direct compelling evidence for this supposition is lacking [22] and is difficult to obtain. A multitude of descriptive studies correlating the presence of resveratrol and protection against oxidative stress have been performed over the past 1-2 years. Almost all of these reports present indirect, correlative evidence. The data clearly support an antioxidant function for resveratrol but do very little to provide mechanistic evidence for its action in vivo. However, a few of the most recent studies are highlighted. 

Evidence suggests that resveratrol may protect against atherosclerosis by enhancing endothelium integrity through the inhibition of protein kinase C (PKC)-mediated signaling [62]. However, resveratrol was also shown to inhibit protein kinase D, a member of the PKC family, but did not affect other PKC isoforms [63, 64]. Furthermore, effects observed in bovine pulmonary artery endothelial cells subjected to stimulated arterial shear stress were also shown to be unrelated to PKC [65]. 

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