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Reporter gene assays proteins

PIAS Proteins. Figure 1 Reporter gene assay with an interferon-y-driven artificial promoter demonstrating the inhibitory transcriptional activity of increasing amounts of PIAS on STAT1 signal transduction. [Pg.977]

A FRET-based reporter gene assay that is now commercially available from Panvera was described by Zlokarneik and co-workers [109]. The assay uses a membrane-permeable substrate (CCE2, a coumarin-fluorescein derivative) for the reporter protein j3-lactamase. By hydrolyzing the substrate, the enzyme disrupts the intramolecular resonance energy transfer between the coumarin and the fluorescein, which changes the fluorescence emission from green at 520 run to blue at... [Pg.636]

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