Zebrafish transgenic

Collas P, Alestrom P (1998) Nuclear localization signals enhance germline transmission of a transgene in zebrafish. Transgenic Res 7 303-309. 

Legler, J., Broekhof, J.L.M., and Brouwer, A. et al. (2000). A novel in vivo bioassay for (xeno-)estrogens using transgenic zebrafish. Environmental Science and Technology 34, 4439 444. 

Immune T-cell-mediated immunosuppression Transgenic zebrafish associated with fluorescent imaging Langenua et al.228... 

Schreurs, R., et al.. Estrogenic activity of UV filters determined by an in vitro reporter gene assay and an in vivo transgenic zebrafish assay. Arch. Toxicol, 76, 257-61, 2002. 

Zebrafish Brachydanio/Danio rerio) embryos can be produced in large numbers and carry sufficient nutrients within the egg sack to allow development within micro plate volumes [52, 53]. Mutation frequency has been estimated indirectly using transgenic fish [54[ and UDS, comet, MNT and alkaline filter elution methods have been used effectively [55]. At present, there has been insufficient study of the model to understand predictivity of mammalian genotoxicity, and none of the methods has been demonstrated at throughputs sufficient for hit and lead screening. 

Amanuma, K., Takeda, H., Amanuma, H. and Aoki, Y. (2000) Transgenic zebrafish for detecting mutations caused by compormds in aquatic environments. Nature Biotechnology, 18, 62-65. 

Foster Yes. I think if we are going to understand what melanopsin is doing a comparative approach will be valuable, and this is why Mark Hankins and I have started to work on zebrafish in parallel with mice. Although the transgenics and gene manipulation is not as sophisticated in fish, there are other things that can be done, particularly at the electrophysiological level. As I discussed in the presentation, Mark Hankins and I are keen to pursue this approach. 

Clark KJ et al (2011) Transgenic zebrafish using transposable elements. Methods Cell Biol 104 137-149... 

Collas, P., Husebye, H. and Alestrom, P. (1996) The nuclear localization sequence of the SV40 T antigen promotes transgene uptake and expression in zebrafish embryo nuclei. Transgenic Res., 5, 451—458. 

Sato Y, Miyasaka N, Yoshihara Y (2005) Mutually exclusive glomerular innervation by two distinct types of olfactory sensory neurons revealed in transgenic zebrafish. J Neurosci 25 4889-4897... 

Carvan, M.J., Sonntag, D.M., Cmar, C.B., Cook, R.S., Curran, M.A. and Miller, G.L. (2001) Oxidative stress in zehrafish cells potential utility of transgenic zebrafish as a deployable sentinel for site hazard ranking. Science of the Total Environment, 274, 183-196. 

---